丹参高密度分子遗传图谱的构建  

Construction of A Genetic Linkage Map in Salvia miltiorrhiza

作　　者：潘玉玲[1] 赵琦[1] 宋振巧[1] 王建华[1] PAN Yu-ling ZHAO Qi SONG Zhen-qiao WANG Jian-hua(State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University,Taian 271018 ,China)

机构地区：[1]山东农业大学农学院/山东省作物生物学重点实验室/作物生物学国家重点实验室,山东泰安271018

出　　处：《中药材》2016年第7期1443-1445,共3页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金(81274012;81001603);山东省自然科学基金(ZR2011HQ007)

摘　　要：目的:以前期研究为依托,利用丹参EST-SSR分子标记法进一步构建丹参高密度遗传图谱,为丹参重要性状相关基因的定位、克隆及分子标记辅助选育新品种等研究提供技术基础。方法:利用紫花丹参74(母本)×白花丹参18(父本)两个品系杂交所得到的F1代植株,对411对引物进行PCR扩增及多态性筛选,结合前期研究得到的标记数据,利用Joinmap 4.0作图软件进行数据整合构建图谱。结果:用2个亲本共筛选了411对EST-SSR引物,共有328对引物有稳定的扩增产物,得到多态性引物164对。结论:最终构建了一张包含150个分子标记的高密度丹参遗传连锁图谱。Objective： On the basis of previous studies,to construct a genetic map of Salvia miltiorrhiza by using EST-SSR primers,to build a platform for positioning important traits relating to genes,the cloning and molecular marker-assisted in breeding of new varieties of Salvia miltiorrhiza. Methods： A total of 411 EST-SSR primers were used for PCR amplification to screen polymorphic markers in F1 mapping population derived from a cross between Salvia miltiorrhiza,two parents of which were the cultivars of ZH74 and BH18. Combined with the molecular marker data of previous studies,Joinmap 4. 0 software was used for map integration. Results： 411EST-SSR primers were screened from two parents,a total of 328 pairs of primers were amplified stable products,164 pairs of polymorphic primers were obtained. Conclusion： A linkage map of Salvia miltiorrhiza with 150 marker high density genetic is constructed.

关 键 词：丹参 遗传连锁图谱 标记 

分 类 号：R282.2[医药卫生—中药学]