UPLC-MS/MS同时测定静脉注射荭草花提取物大鼠血浆中3种成分及其药代动力学研究  

Simultaneous Determination of Protocatechuic Acid,Kaempferol Biotin Glucoside and Quercitrin in Rat Plasma and Pharmacokinetics By UPLC-MS/MS

作　　者：郑林[1,2] 杨武[1] 向文英[1] 孙佳[1,2] 陈思颖[1,2] 李勇军[2,3] ZHENG Lin YANG Wu XIANG Wen-ying SUN jia CHEN Si-ying LI Yong-jun(Guizhou Provincial Key Laboratory of Pharmaceutics School of Pharmacy Engineering Research Center for the Development and Application of Ethnic Medicine and TCM, Ministry of Education, Guizhou Medical University, Guiyang 550004, China)

机构地区：[1]贵州医科大学贵州省药物制剂重点实验室,贵州贵阳550004 [2]贵州医科大学药学院,贵州贵阳550004 [3]贵州医科大学民族药与中药开发应用教育部工程研究中心,贵州贵阳550004

出　　处：《中药材》2016年第7期1574-1577,共4页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金(81360664);贵州省中药现代化科技产业专项基金项目(黔科合中药字[2013]5062号;黔科合重G字[2013]4001)

摘　　要：目的:建立UPLC-MS/MS测定大鼠尾静脉注射荭草花提取物后的大鼠血浆中3种成分(原儿茶酸、山柰素-葡萄糖苷、檞皮苷)含量的分析方法,并研究这3种成分在大鼠体内药代动力学特征。方法:采用Waters BEH C18(50 mm×2.1 mm,1.7μm)色谱柱;流动相为0.1%甲酸乙腈-0.1%甲酸溶液,梯度洗脱;质谱采用电喷雾电离源(ESI);扫描方式为多反应离子监测(MRM)。结果:静脉注射荭草花提取物后原儿茶酸、山柰素-葡萄糖苷、槲皮苷在大鼠体内的半衰期分别为(41.9±12.3)min、(71.3±56.8)min和(90.3±74.8)min,达峰浓度(1.7±0.7)μg/m L、(2.1±0.9)μg/m L和(8.7±3.7)μg/m L。结论:实验结果表明建立的方法快速、准确、灵敏,精密度回收率均符合生物样品的测定要求,适用于原儿茶酸、山柰素-葡萄糖苷、檞皮苷3个成分在大鼠血浆中浓度的测定,3个成分在大鼠体内的药动学特征均符合二室模型。Objective： To develop a sensitive and reliable ultra performance liquid chromatography tandem mass sepctrometry（ UPLC-MS / MS） method for simultaneous determination and pharmacokinetics of protocatechuic acid,kaempferol biotin glucoside and quercitrin in rat plasma,and study their pharmacokinetic characteristics in rats. Methods： Three compounds were simultaneously determined by UPLC-MS / MS on Waters BEH C18（ 50 mm × 2. 1 mm,1. 7 μm） column. The mobile phase was 0. 1% acetonitrile formic acid and0. 1% aqueous formic acid,and programmed in a linear gradient mode. The compounds were ionized in the electrospray ionization ion source of the mass spectrometer and detected in MRM mode. Results： The t1 /2of protocatechuic acid,kaempferol biotin glucoside and quercitrin after intravenous injection were（ 41. 9 ± 12. 3）,（ 71. 3 ± 56. 8） and（ 90. 3 ± 74. 8） min. The Cmaxwere（ 1. 7 ± 0. 7） μg / m L,（ 2. 1 ± 0. 9） μg/m L,（ 8. 7 ± 3. 7） μg/m L. Conclusion： The established method is specific,rapid,accurate and sensitive,and is proved to meet the requirements of biological sample analyses,and is suitable for the concentration determination of protocatechuic acid,kaempferol biotin glucoside and quercitrin in rat plasma,three compounds are all best fitted to the two-compartment open pharmacokinetic model.

关 键 词：UPLC-MS/MS 药动学 荭草花提取物 

分 类 号：R284.1[医药卫生—中药学]