当归补血汤对大鼠肾缺血-再灌注损伤后TLR4/NF-κB信号通路的影响  

Influence of Danggui Buxue Decoction on TLR4/NF-κB Signal Pathway of Rats after Renal Ischemia Reperfusion Injury

作　　者：刘福和[1] 倪文娟 王国康[1] 徐权毅[1] 

机构地区：[1]浙江医药高等专科学校药学院,浙江宁波315100 [2]华东宁波医药有限公司,浙江宁波315806

出　　处：《中国药业》2016年第21期7-10,共4页China Pharmaceuticals

基　　金：浙江省教育厅科研项目,项目编号:Y201534611

摘　　要：目的探讨当归补血汤对大鼠肾缺血-再灌注损伤（RIRI）后TLR4／NF—KB信号通路的影响，为RIRI的作用机制及其干预提供参考。方法将45只SD大鼠随机分为假手术组（Sham组）、缺血-再灌注模型对照组（IR组）、当归补血汤预处理组（IR＋DG组），每组15只。实验前5d，Sham组和IR组以生理盐水10mL／（kg·d）每天灌胃1次，IR＋DG组以当归补血汤10mL／（kg·d）每天灌胃1次。所有大鼠均切除右肾，Sham组仅游离左肾蒂，不夹闭左肾动脉；IR组和IR＋DG组均行RIRI模型制备。比较各组血清肌酐（Cr）、尿素氮（BUN）水平，行肾脏组织形态学检查及肾小管评分，检测肾组织TLR4，NF—KBp65mRNA水平。结果各组均按预定方案完成手术，IR组、IR十DG组大鼠均顺利完成RIRI模型制备；IR组、IR＋DG组血清Cr，BUN水平及肾组织中TLR4mRNA和NF—KBp65mRNA的表达水平均高于Sham组（t=3．72，7．05，P〈0．05或P〈0．01），IR＋DG组血清Cr，BUN水平及肾组织中TLR4mRNA和NF—KBp65mRNA的表达水平均低于IR组（t=4．16～5．37，P〈0．05）；Sham组肾脏呈正常的组织结构，IR组、IR＋DG组均呈程度不-的炎性反应，但IR＋DG组炎性程度轻于IR组；IR组、IR＋DG组肾小管评分均高于Sham组水平（tIR=9．02，tIR＋DG=6．21，P〈0．01），IR＋DG组肾小管评分低于IR组（tIR=5．15，P〈0．05）。结论大鼠RIRI后可能通过TLR4／NF—KB信号通路介导下游级联炎症免疫反应，当归补血汤预处理可能通过抑制TLR4／NF—KB信号通路而缓解RIRI损伤过程中的炎性反应，对大鼠RIRI损伤具有积极的保护作用。Objective To investigate the influence of Danggui Buxue Decoction on TLR4/NF- KB signal pathway of rats after renal is- chemia reperfusion injury（RIRI）, to provide reference for interpretation of its mechanism and intervention. Methods 45 SD rats were randomly divided into the Sham group, IR group, and IR ＋DG group, 15 rats in each group. 5 d before experiment, rats in Sham group and IR group were orally once a day with normal saline by 10 mL/（kg· d）,and rats in IR＋DG group were orally once a day with Danggui Buxue Decoction by 10 mL/（kg · d）. All rats were resected right kidney, rats in Sham group were dissociated left renal pedi- cle, without clamping left renal artery, and rats in IR group and IR ＋ DG group were all given RIRI model preparation. After experi- ment, serum creatinine （Cr） and urea nitrogen （BUN）, renal histology and renal tubule score, TLR4 mRNA and NF- KB p65 mRNA in renal tissue were compared. Results All groups completed operation according to predetermined plan; IR group and IR ＋ DG group suc- cessfully completed RIRI model preparation. Serum Cr and BUN level, TLR4 mRNA and NF- KB p65 mRNA in renal tissue of IR group and IR＋DG group were both higher than that in sham group（t=3.72-7.05, P 〈 0.05 or P 〈 0.01）, and serum Cr and BUN level, TLR4 mRNA and NF-KB p65 mRNA in renal tissue of IR＋DG group were lower than that in IR group（t =4. 16-5.37, P 〈 0. 05）. Sham group＇s kidney showed normal structure, and there were some inflammatory reactions in IR group and IR＋DG group, yet IR ＋DG group＇s inflammatory reaction were slighter than that in IR group. Renal tubules score in IR group and IR ＋DG group were both higher than that in sham group（ tIR = 9.02, tIR＋DC-= 6. 21, P 〈 0. 01）, yet IR ＋ DG group＇s renal tubules score were lower than that in IR group（ tIR= 5.15, P 〈 0. 05）. Conclusion It may mediate downstream cascade inflammatory immune response by TLR4/NF- KB signal pathway after RIRI in rats. Danggui Buxue Decoction pre

关 键 词：缺血-再灌注损伤 信号转导 免疫炎性反应 当归补血汤 干预 大鼠 

分 类 号：R285.5[医药卫生—中药学] R289.5[医药卫生—中医学]