木门百合试管鳞茎培养体系的建立  

Establishment of tissue culture system of bulblets in tube of Lily Conca D'or

作　　者：蔡宣梅[1] 郭文杰[1] 张洁[1] 方少忠[1] 

机构地区：[1]福建省农科院生物技术研究所,福建福州350003

出　　处：《武夷科学》2016年第1期110-114,共5页Wuyi Science Journal

基　　金：福建省科技厅省属公益类科研院所科研专项"食用百合的引种与利用"(2015R1019-5);福建省农科院科技创新团队建设项目(CXTD-2-17)

摘　　要：以百合新品种木门(Conca D'or)的种球鳞片为外植体,进行百合试管鳞茎培养体系研究。结果表明:鳞片诱导的最适培养基为MS+0.5 mg·L^(-1)BA+0.1 mg·L^(-1)NAA+0.1 mg·L^(-1)2,4-D,其诱导率达到95%;膨大培养最适蔗糖浓度为50-70 g·L^(-1);采用分切膨大后的试管鳞茎的鳞片进行增殖培养,平均增殖倍数为7.2;移栽成活率达98%以上。The bulb scales of Lily ＂ Conca D＇or＂ were used as explants to establish the tissue culture system of bulblets in tube. The results showed that the appropriate inductive medium of bulb scales was MS＋0.5 mg ·L-1 BA＋ 0.1 mg · L-1 NAA＋0.1 mg · L-1 2,4-D, with 95% induction rate, and 50-70 g ·L-1 sucrose concentration was appropriate for expanding culture. The bulb scales in tube after split expansion were used to proliferation culture by using the proliferation medium, with 7.2 average multiplication coefficient and 98% transplanting survival rate.

关 键 词：木门百合 试管鳞茎 膨大培养 

分 类 号：S682.2[农业科学—观赏园艺]