鹿茸多肽-FK506-PLGA复合膜修复周围神经损伤的实验研究  

The research of pilose antler polypeptide-FK506-PLGA composite membrane in the repair of peripheral nerve injury

作　　者：崔军[1] 莫忆南[1] 

机构地区：[1]沈阳医学院附属中心医院,辽宁沈阳110024

出　　处：《中国医药科学》2016年第17期30-32,共3页China Medicine And Pharmacy

摘　　要：目的 探究鹿茸多肽-FK506-PLGA复合膜在周围神经损伤修复中的应用价值。方法 取4~6周龄雄性SD大鼠72只制备坐骨神经损伤模型,随机分为4组各18只,分别对神经吻合口部位行鹿茸多肽-FK506-PLGA复合膜包绕（A组）、鹿茸多肽-PLGA复合膜包绕（B组）、FK506-PLGA复合膜包绕（C组）以及直接端端吻合（D组）,比较各组大鼠术后2、4、6周小腿三头肌诱发电位恢复率及其再生有髓纤维计数（n）、直径（mm）及截面积恢复率（%）情况。结果 术后各时点A组大鼠电位恢复率明显优于B、C、D组（P均〈0.05）,且B、C组大鼠电位恢复率也较对照组D组具有明显改善。术后2、4、6周时,A组大鼠再生有髓纤维数、直径及截面积恢复率均明显优于B组、C组、D组（P均〈0.05）,D组的再生有髓纤维数、直径及截面积恢复率较B、C组均明显偏低,差异有统计学意义（P〈0.05）。结论 鹿茸多肽-FK506-PLGA复合膜可有效促进周围神经缺损的再生和修复,在保证外周神经有效营养供给的同时提供良好的生物相容性。Objective To explore the application value of pilose antler polypeptide - FK506 - PLGA composite membrane in the repair of peripheral nerve injury. Methods 72 male SD rats （4-6 weeks） were selected to build sciatic nerve injury model, and they were divide into 4 groups, with 18 cases in each. Rats in group A were treated with nerve anastomosis with pilose antler polypeptide - FK506 - PLGA composite membrane, rats in group B were treated with deer antler polypeptide - PLGA composite film bag, rats in group C were treated with FK506 around -PLGA composite membrane, and rats in group D were treated with end to end anastomosis. The leg triceps evoked potential recovery rate, regenerative myelinated fibers count （n）, diameter （mm） and cross-sectional area recovery rate （%） after 2, 4, 6 weeks in different groups were compared. Results The potential recovery rate in group A in different points were significantly better than that in group B, C and D（P 〈 0.05）,and the potential recovery rate in group B and C were better than that in group D. At 2, 4, 6 weeks postoperatively, the regenerative myelinated fibers count, diameter and cross-sectional area recovery rate in group A were significantly better than those in group B, C and D （P 〈 0.05）, and the regenerative myelinated fibers count, diameter and cross-sectional area recovery rate in group D were significantly lower than those in group B and C. Conclusion Pilose antler polypeptide - FK506 - PLGA composite membrane can effectively promote the regeneration of the peripheral nerve defect effectively, and support a good biocompatibility as well as effective nutrient supply.

关 键 词：鹿茸多肽-FK506-PLGA复合膜 周围神经损伤 

分 类 号：R285.5[医药卫生—中药学]