血管紧张素原基因M235T多态性与糖尿病慢性肾脏疾病的相关性研究  

The relationship between angiotensinogen gene M235T polymorphism and diabetic chronic kidney disease

作　　者：郭毅飞[1] 李雪锋[1] 李敏[1] 王娜娜[1] 

机构地区：[1]十堰市太和医院内分泌代谢病科,湖北十堰442000

出　　处：《临床和实验医学杂志》2016年第21期2081-2084,共4页Journal of Clinical and Experimental Medicine

基　　金：湖北省教育厅科学技术研究计划重点项目(No:D20112105)

摘　　要：目的探讨血管紧张素原基因M235T是否影响糖尿病肾病的发生。方法收集收治的糖尿病患者300例,根据尿蛋白尿和尿肌酐的比值(ACR)分为三组:单纯糖尿病组(ACR<30 mg/g),微量蛋白尿组(30 mg/g<ACR<300 mg/g),大量蛋白尿组(ACR>300 mg/g)。另收集健康体检人群100例作为对照组。检测各组常规指标,采用聚合酶链反应-限制性片断长度多态性技术进行M235T多态性位点多态性检测。结果对照组和单纯糖尿病组、微量蛋白尿组、大量蛋白尿组基因型分布均符合Hardy-Weinberg平衡定律;对照组和糖尿病组M235T基因型分布和等位基因频率无统计学差异(P>0.05);单纯糖尿病组、微量蛋白尿组和大量蛋白尿组M235T基因型分布和等位基因频率无统计学差异(P>0.05)。结论在本区域人群中,血管紧张素原基因M235T多态性与糖尿病肾病无相关性。Objective Explore whether the angiotensinogen gene M235T polymorphism could affect the occurrence of diabetic nephropathy. Methods 300 cases of diabetes patients were divided into three groups according to the ratio of urinary proteinuria and urine creatinine （ACR） ： DM group, microalbuminuria group and macroalbuminuria group. 100 cases of healthy people were selected as control group. Conventional indicators were detected. M235T polymorphism were detected by polymerase chain reaction -restriction fragment length polymorphism technique. R^ults Normal group and the diabetic group, microalbuminuria, macroalbuminuria group genotypes were in accordance with Hardy - Weinberg equilibrium law. M235T genotype distribution and allele frequency in normal group and diabetic group has no significant difference （ P 〉 0.05）. M235T genotype dis- tribution and allele frequency in DM group, microalbuminuria and macroalbuminuria group has no significant difference. Conclusion There is no correlation between angiotensinogen gene M235T polymorphism and diabetic nephropathy of the peple in the region.

关 键 词：糖尿病肾病 血管紧张素原基因M235T 基因多态性 相关性 

分 类 号：R587.2[医药卫生—内分泌] R692.9[医药卫生—内科学]