电针对WKY模型大鼠抑郁样行为及缰核β-CaMK Ⅱ蛋白的影响  

Effect of Electro-acupuncture on Depressive-like Behavior and β-CaMK Ⅱ of Habenula in WKY Rats Model of Depression

作　　者：陈泽钦[1] 董波[1] 李蔚[2] 黄丽[1] 韩栩珂 徐世芬[1] 

机构地区：[1]上海中医药大学附属市中医医院,上海200071 [2]新疆医科大学,新疆乌鲁木齐840000

出　　处：《中医药导报》2016年第21期22-26,共5页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：上海市教委科研创新重点项目(2014zz116);上海市卫生和计划生育委员会项目(ZY3-CCCX-3-3022)

摘　　要：目的:观察电针对WKY抑郁模型大鼠行为学及缰核β-CaMK Ⅱ蛋白的影响。方法:将24雄性WKY大鼠随机分为电针组(EA)、假针组(sham EA)、模型组(model)。Wistar雄性大鼠8只设为正常组(normal)。21 d后通过旷场实验、糖水偏好实验、强迫游泳实验对大鼠进行行为学评价,用免疫荧光法检测大鼠缰核中β-CaMK Ⅱ蛋白表达。结果:与正常组比较,模型组旷场实验的水平运动、垂直运动和蔗糖水摄入比明显下降(P<0.05),强迫游泳的不动时间和缰核β-CaMK Ⅱ表达均增加(P<0.05)。与假针组比较,电针组旷场实验的水平运动和垂直运动、蔗糖水摄入比均明显增加(P<0.05),强迫游泳的不动时间和缰核β-CaMK Ⅱ蛋白表达减少(P<0.05)。结论:电针对WKY抑郁模型大鼠的行为学核心症状有明显的改善作用,能明显降低缰核中β-CaMK Ⅱ蛋白表达。这可能是电针治疗抑郁症的机制之一。Objective： To observe the effects of eleetro-acupuncture（EA） on depressive-like behavior and β-CaMKⅡ protein expression of habenula in WKY rat model. Methods： The 24 male WKY rats were randomly divided into EA, sham, model groups. 8 Wistar rats were divided into normal group. The behaviors were evaluated by open fieht test, Sucrose preference test and force swimming test alter 21 days, the expression of β-CaMKⅡ was detected by immunofluorescence. Results： Compared with the control group, model group decreased signifi- cantly in open fieht test and sucrose preference test （P〈0.05）, and increased significantly in immobile time of tbrce swimming test and the β-CaMKⅡ expression of habenula （P〈0.05）. Compared with sham EA group, EA group increased significantly in open field test and sucrose preference test （P〈0.05）,and decreased significantly in immobile time of force swimming test and the β-CaMKⅡ expression of habenula （P〈0.05）. Conclusions： Electroacupuncture can improve the WKY rats＇ depressive-like behaviors and decrease the expression of β-CaMKⅡ protein in habenula. It may be one of the mechanisms of electro-acupuncture on depression.

关 键 词：电针 抑郁症 缰核 β-CaMKⅡ蛋白 WKY大鼠 

分 类 号：R285.5[医药卫生—中药学]