微小RNA-21在感染性早产胎盘组织中的表达及意义  

Expression and significance of miR-21 in placental tissues of rats' infectious preterm birth model

作　　者：段丽君[1] 孙晓彤[1] 单龙[1] 刘晓雁[1] 贺小燕[1] 杨雪萍[1] 秦艳彬 王金羊[1] 黄丽辉[1] 彭彩霞[1] 杨燕[1] 

机构地区：[1]甘肃省人民医院妇产科,甘肃兰州730000

出　　处：《兰州大学学报（医学版）》2016年第5期21-25,共5页Journal of Lanzhou University（Medical Sciences）

基　　金：国家自然科学基金项目(81560254);甘肃省卫生行业科研项目(GWGL2014-59)

摘　　要：目的通过观察微小RNA-21(mi R-21)在孕鼠胎盘组织的表达,探讨感染性早产的发病机制。方法建立脂多糖诱导的感染性早产模型。孕鼠随机分为对照组、模型组和干预组。对照组腹腔注射生理盐水0.1 m L,模型组腹腔注射脂多糖0.1 m L,干预组腹腔注射脂多糖0.1 m L+20 mg/kg mi R-21模拟物(化学合成的mi R-21)。应用实时荧光定量PCR检测各组胎盘组织中mi R-21的表达,用生物信息学分析方法预测mi R-21的靶点,用免疫组织化学法检测胎盘组织中核因子-κB(NF-κB)蛋白的表达。结果与对照组比较,mi R-21表达在模型组胎盘组织中显著降低,而NF-κB蛋白表达明显升高(P<0.05)。NF-κB是mi R-21的预测靶点,mi R-21模拟物能够下调胎盘绒毛膜组织中NF-κB蛋白表达。结论 mi R-21表达缺失可能参与了感染性早产的发病机制,对以后设计以mi R-21为靶点的特异性药物可能有重要的意义。Objective To investigate the miR-21expression in placental tissues in rat model of infectious preterm birth （IPTB） and further elucidate the mechanism of IPTB. Methods An experimental rat model of IPTB was established by intraperitoneal （IP） injection of LPS. Pregnant rats were randomly divided into three groups： normal control group （NC group, n=10）, model group （LPS group, 0.1 mg/mL LPS 0.1 mL, IP, n=10） and miR-21 mimics group（LPS 0.1 mL＋ 20 mg/kg miR-21 mimics, IP, n=1 0）, the expression of miR-21 in placental tissues were detected by RT-PCR. Bioinformatics analysis predicted the targets of miR-21. NF-κB protein expression was detected by immunohistochemistry （IHC）. Results Compared with NC group, significantly decreased miR-21 expression was （P 〈 0.05）, and significantly increased NF-κB protein expression were foundin LPS group （P 〈 0.05）. Interestingly, NF-κB was a predicted target of miR-21 by bioinformatic analysis. MiR-21 mimics can downregulate NF-κB protein expression （P 〈 0.05）. Conclusion The loss of miR-21 expression may be involved in the pathogenesis of IPTB, and it may be of great significance for the fu- ture design of specific drugs targeting miR-21.

关 键 词：微小RNA 感染性早产 实时荧光定量PCR 生物信息学分析 

分 类 号：R271.4[医药卫生—中医妇科学]