不同浓度磺胺二甲嘧啶对中国对虾APND、ECOD和GST活性的影响  

Effect of Sulfamethazine on APND,ECOD and GST Activities of Fenneropenaeus chinensis

作　　者：孙铭[1,2] 翟倩倩[2] 常志强[2] 葛倩倩[1,2] 李健[2,3] 赵法箴[2,3] 

机构地区：[1]中国海洋大学水产学院,山东青岛266003 [2]中国水产科学研究院黄海水产研究所,山东青岛266071 [3]青岛海洋科学与技术国家实验室海洋渔业科学与食物产出过程功能实验室,山东青岛266235

出　　处：《中国海洋大学学报（自然科学版）》2016年第10期16-23,共8页Periodical of Ocean University of China

基　　金：国家虾产业技术体系专项项目(CARS-47);山东省自主创新专项项目(2013CXC80202);国家高技术研究发展计划项目(2012AA10A409);2013年中国水产科学研究院院级基本科研业务费专项项目(2013A1102);公益性行业(农业)科研专项项目(201103034)资助~~

摘　　要：为研究药物代谢酶对磺胺二甲嘧啶作用的响应及其在中国对虾代谢磺胺二甲嘧啶过程中的作用,以50、100和150mg/kg(干重)磺胺二甲嘧啶连续投喂中国对虾5d,于投喂后的第1、2、3、4、5天和停止投喂药物的第1、2、3、4、5、7、10天取样,测定中国对虾肝胰腺、鳃和血清中的氨基比林-N-脱甲基酶(Aminopyrine-N-demethylase,APND)、7-乙氧基香豆素-O-脱乙基酶(7-ethoxycoumarin-O-Deethylase,ECOD)和谷胱甘肽还原转移酶活性(Glutathione-S-transferase,GST)的含量。研究表明:磺胺二甲嘧啶对中国对虾肝胰腺、鳃和血清中I相药物代谢酶(APND、ECOD)活性的抑制作用均具有剂量依赖性。投喂药物期间,酶活性呈逐渐下降趋势,而停止投喂后,酶活性呈逐渐上升趋势,至实验结束时均恢复至对照组水平。在整个实验周期内,高浓度组的血清GST活性呈现抑制作用,其它浓度磺胺二甲嘧啶组的各组织中GST活性呈现诱导作用。磺胺二甲嘧啶对中国对虾肝胰腺和鳃ECOD活性发生抑制作用的时间较ANPD晚。结果表明:中国对虾对磺胺二甲嘧啶的代谢,一方面通过抑制I相代谢酶的活性,抑制药物的活化,另一方面通过诱导II相代谢酶的活性,加速药物的解毒与代谢。在实际生产中,磺胺类药物通常会与增效剂甲氧苄啶合用以提高药效,应考虑磺胺二甲嘧啶对药物代谢酶的抑制作用,以减轻甲氧苄啶可能在体内的蓄积和毒效作用。The antimicrobial sulfamethazine is widely used in aquaculture for the prevention and treatment of bacterial diseases.Residues of sulfamethazine have been detected in aquatic environments and edible tissues of aquaculture fish and shrimps at relatively low,but detectable concentrations,which is a potential risk to human health.It is necessary to understand the mechanism of its drug metabolism.In order to study the response of drug metabolism enzyme to sulfamethazine and their functions in the metabolism of sulfamethazine,the shrimp were fed with medicated feed at 50,100 and 150 mg/kg（BW）daily,and sampled at day 1,2,3,4,5during the drug exposure period and day 1,2,3,4,5,7,10 after drug exposure.Aminopyrine-N-demethylase（APND）,7-ethoxycoumarin-O-deethylase（ECOD）and glutathione-S-transferase（GST）in hepatopancrease,gill and serum were determined.Results showed that the effect of sulfamethazine on the activities of Phase I metabolism enzyme was dose-dependent.The inhibition of sulfamethazine on APND and ECOD activities increased with dose.APND and ECOD activities were significant lower than those of control,which decreased during the drug exposure while increased gradually after exposure.For APND,the significant difference was occurred earlier at 150mg/kg（BW）than at 50 and 100mg/kg（BW）.The inhibition of sulfamethazine to APND in hepatopancrease,gill and serum was the highest at day 5during exposure.The inhibition of sulfamethazine to ECOD in hepatopancrease and gill was not occurred immediately after the drug administration,which was occurred after drug administration at 50 and 100mg/kg（BW）while the inhibition of sulfamethazine to ECOD in serum was occurred immediately after drug administration.Different concentrations of SM2 can induce GST activity in hepatopancrease and gills significantly compared to the control in a dose-dependent manner.In 50 and 100mg/kg（BW）,GST activity in serum was inhibited on the first 2days of the exposure followed by an induction,and peaked on day 5of the ex

关 键 词：磺胺二甲嘧啶 中国对虾 氨基比林-N-脱甲基酶(APND) 7-乙氧基香豆素-O-脱乙基酶(ECOD) 谷胱甘肽还原转移酶(GST) 

分 类 号：S948[农业科学—水产养殖] S943[农业科学—水产科学]