放射性脑损伤大鼠脑皮层组织中胶质原纤维酸性蛋白表达及微血管通透性观察  

Changes of capillary permeability and GFAP expression after radiation-induced brain injury in rats

作　　者：孙熠[1] 陈泊霖 金学隆[1] 

机构地区：[1]天津医科大学,天津300070

出　　处：《山东医药》2016年第39期20-22,共3页Shandong Medical Journal

基　　金：国家科技计划项目国际科技合作专项子项目(2011DFA30550)

摘　　要：目的观察放射性脑损伤(RIBI)大鼠脑皮层胶质原纤维酸性蛋白(GFAP)及微血管通透性。方法SPF清洁级成年健康雄性SD大鼠48只分为A、B、C组和对照组各12只。A、B、C组采用CT大脑局部照射造成RIBI,2 Gy/次,共照射3次,总照射剂量6 Gy。对照组仅腹腔注射麻醉大鼠,不行大脑局部照射。分别于照射7、14、28 d时取各组部分大鼠,断头处死后取脑组织,采用免疫组化法检测各组脑皮层GFAP。于照射7、14、28 d时取各组部分大鼠,断头处死后取脑组织,采用微循环显微仪观察各组损伤脑皮层微血管通透性。结果 A、B、C组和对照组的血管通透性分别为0.70±0.082、0.58±0.019、0.54±0.058、0.39±0.073,组间比较,P均<0.05。A、B、C组和对照组脑皮层GFAP的IOD值分别为145 006±5 266.8、101 250±9 034.8、68 313±7 633.4、17 617±5 870.7,组间比较,P均<0.05。结论 RIBI大鼠损伤脑皮层存在GFAP高表达,GFAP的表达水平与微血管通透性的变化趋势相一致,GFAP可能参与了RIBI的发生发展。Objective To observe the dynamic changes of capillary permeability and the expression of glial fibrillary acid protein（ GFAP） in cerebral cortex after radiation-induced brain injury（ RIBI） in rats. Methods Forty-eight adult male healthy SD rats were randomly divided into groups A,B,C and the control group,12 in each. RIBI models of groups A,B and C were established by CT scanning with a whole dose of 6 Gy,2 Gy/time for 3 times. Animals in the control group were subjected to the same surgical procedure without cranial irradiation. The expression of GFAP in the parietal cortex was analyzed at different time points（ 7,14 and 28 h） by immunohistochemistry（ IHC）,and the capillary permeability was evaluated by microscope vascular camera device and microcirculation video recording system. Results The capillary permeability in the groups A,B and C was 0. 70 ± 0. 082,0. 58 ± 0. 019,0. 54 ± 0. 058 and 0. 39 ± 0. 073,which was significantly higher than that in the control group（ 0. 39 ± 0. 073）（ all P〈0. 05）. Similarly,IHC indicated that the expression levels of GFAP in the groups A,B,C were 145 006 ± 5 266. 8,101 250 ± 9 034. 8 and 68 313 ± 7 633. 4,which were significantly higher as compared with that of the control group（ 17 617 ± 5 870. 7）,（ all P〈0. 05）. Conclusion The level of GFAP is high in the brain cortex of RIBI rats,which is consistent with the change of capillary permeability after RIBI,and this strongly indicates that GFAP may participate the occurrence and development of RIBI.

关 键 词：放射性脑损伤 胶质原纤维酸性蛋白 微血管通透性 动物实验 

分 类 号：R651.1[医药卫生—外科学]