异丙酚对内脏痛大鼠PSDC神经元NK-1受体内化和CREB磷酸化的影响  

Effects of propofol on NK-1 receptor internalization and CREB phosphorylation in spinal postsynaptic dorsal column neurons of rats with visceral pain

作　　者：利雪阳 王云[1] 郭瑞娟[2] 赵玉洁 谢芳[1] 时蓉[1] 吴安石[1] 岳云[1] 

机构地区：[1]首都医科大学附属北京朝阳医院麻醉科,100020 [2]首都医科大学附属北京友谊医院麻醉科,100050 [3]北京清华长庚医院麻醉科,102218

出　　处：《中华麻醉学杂志》2016年第8期965-967,共3页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金（30801073,81400909,81571065）

摘　　要：目的 评价异丙酚对内脏痛大鼠脊髓突触后背柱（PSDC）神经元神经激肽-1（NK-1）受体内化和环磷腺苷效应元件结合蛋白（CREB）磷酸化的影响.方法 成年雄性SD大鼠,体重300～ 350 g,行鞘内置管和PSDC神经元逆行预标记术.取鞘内置管成功的大鼠30只,采用随机数字表法分为3组（n=10）：对照组（C组）、内脏痛组（VP组）和异丙酚组（P组）.VP组和P组直肠给予10％辣椒素溶液50 μl制备内脏痛模型,C组直肠给予生理盐水50μl;直肠给药前10 min时C组和VP组鞘内注射二甲基亚砜10μl,P组鞘内注射异丙酚20 μg（用二甲基亚砜稀释至10μl）.记录直肠给药后30 min内内脏疼痛评分,然后深麻醉状态下取腰骶段脊髓组织,采用免疫荧光组化检测脊髓PSDC神经元NK-1受体和磷酸化CREB（p-CREB）的表达水平.结果 与C组比较,VP组和P组内脏痛评分升高,脊髓PSDC神经元NK-1受体和p-CREB的表达上调（P＜0.05或0.01）;与VP组比较,P组内脏痛评分降低,脊髓PSDC神经元NK-1受体和p-CREB的表达下调（P＜0.01）.结论 异丙酚减轻大鼠内脏痛的机制可能与抑制PSDC神经元NK-1受体内化和CREB磷酸化有关.Objective To evaluate the effects of propofol on the internalization of neurokinin-1 （NK-1） receptors and phosphorylation of cyclic adenosine monophosphate response element-binding protein （CREB） in spinal postsynaptic dorsal column （PSDC） neurons of rats with visceral pain.Methods Adult male Sprague-Dawley rats,weighing 300-350 g,in which intrathecal catheters were implanted and retrograde prelabeling of PSDC neurons was performed,were used in the study.Thirty rats in which intrathecal catheters were successfully implanted were randomly divided into 3 groups （n=10 each） using a random number table：control group （group C）,visceral pain group （group VP）,and propofol group （group P）.Visceral pain was induced by injection of 10% capsaicin 50 μl via the rectum in VP and P groups,and normal saline 50 μl was given via the rectum in group C.At 10 min before rectal administration,dimethyl sulfoxide 10 μl was intrathecally injected in C and VP groups,and propofol 20 μg （diluted to 10 μl in dimethyl sulfoxide） was intrathecally injected in group P.Visceral pain was assessed and scored within 30 min after rectal administration.The rats were then anesthetized and sacrificed,and the lumbar segments （L3-6） of the spinal cord were removed for determination of the expression of NK-1 receptors and phosphorylated CREB （p-CREB） in spinal PSDC neurons by immunofluorescence histochemistry.Results Compared with group C,the visceral pain score was significantly increased,and the expression of NK-1 receptors and p-CREB in spinal PSDC neurons was significantly up-regulated in VP and P groups （P〈0.05 or 0.01）.Compared with group VP,the visceral pain score was significantly decreased,and the expression of NK-1 receptors and p-CREB in spinal PSDC neurons was significantly down-regulated in group P （P〈 0.01）.Conclusion The mechanism by which propofol attenuates visceral pain is probably relatcd to inhibition of NK-1 receptor internalization and CREB phosphorylation in spinal PSDC neu

关 键 词：二异丙酚 疼痛 内脏 受体 神经激肽1 CAMP反应元件结合蛋白质 脊髓 突触 神经元 

分 类 号：R614[医药卫生—麻醉学]