右美托咪定对脂多糖诱导小胶质细胞炎性反应时NF-κB表达的影响  

Effect of dexmedetomidine on NF-κB expression during lipopolysaccharide-induced inflammatory responses in microglias

作　　者：张小宝[1] 闫芳[2] 朱品[1] 冯继英[1] 栾恒飞[1] 武勇[1] 赵志斌[1] 

机构地区：[1]连云港市第一人民医院麻醉科,222000 [2]南京医科大学康达学院生物化学教研室,连云港市222006

出　　处：《中华麻醉学杂志》2016年第8期1007-1009,共3页Chinese Journal of Anesthesiology

基　　金：连云港市科学技术局课题资助（SH1402）

摘　　要：目的 评价右美托咪定对脂多糖诱导小胶质细胞炎性反应时NF-κB表达的影响.方法 正常培养的BV-2小胶质细胞,采用随机数字表法分为3组（n=15）：对照组（C组）、脂多糖组（L组）和右美托咪定组（D组）.C组加入无血清培养基培养24 h;L组加入脂多糖（终浓度1μg/ml）,孵育24 h;D组加入右美托咪定（终浓度0.1 μmol/L）,30 min后加入脂多糖（终浓度1μg/ml）,孵育24 h.采用硝酸酶还原法测定上清液一氧化氮（NO）浓度,采用RT-PCR法测定上清液诱导型一氧化氮合酶（iNOS） mRNA表达,采用Western blot法测定细胞NF-κB表达.结果 与C组比较,L组和D组上清液NO浓度升高,iNOS mRNA和细胞NF-κB表达上调（P＜0.01）;与L组比较,D组上清液NO浓度降低,iNOS mRNA和细胞NF-κB表达下调（P＜0.01）.结论 右美托咪定可通过抑制NF-κB活性减轻脂多糖诱导小胶质细胞的炎性反应.Objective To evaluate the effect of dexmedetomidine on nuclear factor kappa B （NF-κB） expression during lipopolysaccharide （LPS）-induced inflammatory responses in microglias.Methods Normally cultured BV-2 microglias were randomly divided into 3 groups （n =15 each） using a random number table：control group （group C）,LPS group （group L） and dexmedetomidine group （group D）.Microglias were incubated in serum-free culture media for 24 h in group C.LPS （final concentration 1 μg/ml） was added,and microglias were incubated for 24 h in group L.In group D,dexmedetomidine （final concentration 0.1 μmol/L） was added,30 min later LPS （final concentration 1 μg/ml） was added,and then microglias were incubated for 24 h.The concentration of nitric oxide （NO） in the supernatant was measured by nitrate reductase method.The expression of inducible nitric oxide synthase （iNOS） mRNA in the supernatant was detected by real-time polymerase chain reactiom NF-κB expression in microglias was detected by Western blot analysis.Results Compared with group C,the concentration of NO was significantly increased,and the expression of iNOS mRNA and NF-κB was significantly up-regulated in L and D groups （P〈0.01）.Compared with group L,the concentration of NO was significantly decreased,and the expression of iNOS mRNA and NF-κB was significantly down-regulated in group D （P〈0.01）.Conclusion Dexmedetomidine attenuates LPS-induced inflammatory responses in microglias through inhibiting NF-κB activity.

关 键 词：右美托咪啶 脂多糖类 小神经胶质细胞 炎症 

分 类 号：R614[医药卫生—麻醉学]