紫外分光光度法测定黄芩多糖的含量  

Determination of polysaccharide in Scutellaria Radix by UV method

作　　者：朱鹤云[1] 关皎[1] 王佳林[1] 姜吉[1] 郝乘仪[1] 冯波[1] ZHU Heyun GUAN Jiao WANG Jialin JIANG Ji HAO Chengyi FENG Bo(College of Pharmacy, Jilin Medical University, Jilin City, Jilin Province, 132013, China)

机构地区：[1]吉林医药学院药学院,吉林吉林132013

出　　处：《吉林医药学院学报》2016年第5期334-335,共2页Journal of Jilin Medical University

基　　金：吉林省卫生厅科研课题(2013Z008);吉林省中医药管理局科研课题(2015Z726)

摘　　要：目的采用紫外分光光度法测定黄芩中多糖的含量。方法用超声-沸水浴提取黄芩中的多糖,以葡萄糖为标准品,在490 nm处测定其中多糖的含量。结果测得黄芩中多糖的平均含量为12.8%,平均回收率为99.0%。结论该方法结果准确,操作简便,具有良好的重现性和稳定性,适合黄芩药材中多糖含量测定。Objective To develop an UV method for the ods Polysaecharide was extracted from Scutellaria Radix determination of polysaccharide in Scutellaria Radix. Meth- by ultrasonic and boiling water bath and determined at 490 nm,while glucose was used as standard substance. Results The average content of polysaccharide was 12.8% ,and the average recovery was 99.0%. Conclusion The developed method is simple, accurate with high repeatability and stability;it is suitable for the determination of polysaccharide in Scutellaria Radix.

关 键 词：黄芩 多糖 紫外分光光度法 

分 类 号：R284.2[医药卫生—中药学]