Ⅰ～Ⅱ期糖尿病肾病大鼠模型扩散张量成像的初步研究  

A preliminary study in stage Ⅰ and Ⅱ of diabetic nephropathy rats using diffusion tensor imaging MRI at 3.0T

作　　者：胡小艳[1] 邬颖华[1] 郭亮[2] 匡敏[1] 王钒[3] HU Xiao-yan WU Ying-hua GUO Liang KUANG Min WANG Fan(． Chengdu University of TCM, Chengdu 610075, China China Medical University, Shenyang 110001, China Department of Radiology, the Affiliated Hospital of Chengdu University of TCM, Chengdu 610075, China)

机构地区：[1]成都中医药大学,四川成都610075 [2]中国医科大学,辽宁沈阳110001 [3]成都中医药大学附属医院放射科,四川成都610075

出　　处：《中国临床医学影像杂志》2016年第11期795-798,共4页Journal of China Clinic Medical Imaging

基　　金：四川省教育厅自然科学重点项目(编号:132A0285)

摘　　要：目的：探讨Ⅰ~Ⅱ期糖尿病肾病大鼠模型磁共振张量成像（DTI）表现及变化规律。材料与方法：20只SD雄性大鼠，按随机数字表法将大鼠分为2组，实验组14只，实际存活9只，共18个肾脏，对照组6只，共12个肾脏；实验组给予1%链脲佐菌素腹腔注射造模，对照组给予同等剂量的柠檬酸缓冲液（pH 4.5）；2周后对两组大鼠行常规MRI检查及DTI检查，在6个非共线方向上施加扩散敏感梯度场，b值取0、600 s/mm2；并留取血尿标本测量尿微量白蛋白、血肌酐；取双肾行病理检查；由两名经验丰富的放射科医师盲法观察及测量双侧肾脏皮质、髓质兴趣区的ADC值和FA值。结果：实验组大鼠血糖明显升高，两组血肌酐、尿微量白蛋白均正常；实验组动物大鼠双肾病理表现为Ⅰ~Ⅱ期DN损害，对照组大鼠双肾病理均正常；实验组大鼠皮、髓质ADC值较对照组增大，差异有统计学意义（P〈0.05）；实验组皮、髓质FA值比较，差异有统计学意义（P〈0.05）；实验组皮、髓质FA值与对照组皮、髓质FA值比较，差异无统计学意义（P〉0.05）。结论：DTI成像的ADC值和FA值能够在一定程度上，较血尿生化指标更能反映早期DN的病理改变。Objective： To prospectively assess the manifestation of stage Ⅰ and Ⅱ diabetic nephropathy rats using diffusion tensor imaging（DTI） MRI at 3.0T. Materials and Methods： Twenty male SD rats were randomly divided into 2 groups according to the random number table. Fourteen rats were in the experimental group, the final survival of 9 rats with 18 kidneys, and 6 rats were in the control group with 12 kidneys. The experimental group was treated with 1% streptozotocin, and the control group was given the same dose of citrate buffer solution. Two weeks later, both of the two groups of rats underwent conventional MRI and DTI examination with b value of 0 and 600 s/mm2（diffusion directions=6）, and the serum creatinine and microalbuminuria were measured and the pathological change of all kidneys were examined. Mean apparent diffusion coefficient（ADC） and fractional anisotropy（FA） were determined for the cortex and the medulla by two experienced radiologists blinded. Results： The blood glucose of experimental group was significantly increased, and the serum creatinine and microalbuminuria in both of the two groups of rats were normal. The pathological changes of the kidneys in the experimental group were stage Ⅰ and Ⅱ diabetic nephropathy expression, and the control group was normal. The ADC values of cortex and medulla were increased in the experimental group compared with the control group and the difference was statistically significant（P〈0.05）. In the experimental group, the difference of FA values were statistically significant between cortex and medulla（P〈0.05）. While there were no significant differences in the FA values of the cortex and medulla between two groups（P〉0.05）. Conclusion： The ADC value and FA value of DTI imaging maybe be more able to reflect the pathological changes than the biochemical indicator in the early stage of diabetic nephropathy.

关 键 词：糖尿病肾病 磁共振成像 

分 类 号：R587.2[医药卫生—内分泌] R445.2[医药卫生—内科学]