一例鸭1型甲肝病毒和11型鸭疫里默氏杆菌混合感染的诊断及其病原的分离鉴定  

Diagnosis of Duck Hepatitis Virus Type 1 and Serotype 11 Riemerella anatipestifer Mixed Infection and Isolation and Identification of Pathogens

作　　者：陈翠腾[1] 程龙飞[1] 傅光华[1] 万春和[1] 刘荣昌[1] 傅秋玲[1] 陈珍[1] 朱春华[1] 陈红梅[1] 施少华[1] 黄瑜[1] CHEN Cui-teng CHENG Long-fei FU Guang-hua WAN Chun-he LIU Rong-chang FU Qiu-ling CHEN Zhen ZHU Chun-hua CHEN Hong-mei SHI Shao-hua HUANG Yu~(Fujian Key Laboratory of Poultry Disease Prevention and Control, Fujian Animal Disease Control Technology Development Center, Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Science, Fuzhou 350013, China)

机构地区：[1]福建省农业科学院畜牧兽医研究所,福建省禽病防治重点实验室,福建省畜禽疫病防治工程技术研究中心,福州350013

出　　处：《中国畜牧兽医》2016年第11期3053-3058,共6页China Animal Husbandry & Veterinary Medicine

基　　金：福建省农业科学院青年人才创新基金(2014CX-4);现代农业产业体系建设专项资金(CARS-43);新世纪“百千万人才工程”国家级人选科研补助基金(NCNCMTPC-2009);福建省优良蛋鸭品种与设施化养殖创新产业化工程项目;福建省畜禽疫病防控技术重大研发平台(2014N2003)

摘　　要：为确定引起2015年6月底福州仓山区某一养鸭场10日龄左右麻鸭发病死亡的病原,无菌采取病死鸭的肝脏、脾脏、脑等组织,经病毒分离、细菌分离纯化、PCR检测、血清学检测、药物敏感性试验,结果检测并分离到鸭1型甲肝病毒和11型鸭疫里默氏杆菌,该鸭1型甲肝病毒分离株与MPZJ1206株和GD株同源性最高,均为98.7%。结果表明,该病例为鸭甲肝病毒1型和11型鸭疫里默氏杆菌混合感染。To determine the pathogens that caused the death of the 10-day-old sheldrakes of a outbroke epidemic duck farm at the end of June 2015,at Cangshan District in Fuzhou,the liver,spleen and brain of dead sheldrakes were collected,and then tested by virus isolation,isolation and purification of bacteria,PCR detection,serology and drug susceptibility test.The results showed duck hepatitis virus type 1and 11 type Riemerella anatipestifer were detected and isolated,and the isolated duck hepatitis virus type 1had the highest homology with MPZJ1206 strain and GD strain,and the percentage of homology were 98.7%.Laboratory isolation and identification of pathogen determined that the case was duck hepatitis virus type 1and serotype 11 Riemerella anatipestifer mixed infection.

关 键 词：鸭甲肝病毒1型 11型鸭疫里默氏杆菌 分离鉴定 

分 类 号：S858.32[农业科学—临床兽医学]