二花脸FSH的高量表达对大白公猪睾丸的影响  

Influence of Erhualian FSH High-level Expression on the Testes of Large White Boars(Sus scrofa)

作　　者：徐盼[1,2] 李完波[1] 蒋凯[1] 杨强[1] 李龙云[1] 乔传民 龚焕发 任军[1] 幸宇云[1] XU Pan LI Wan-Bol JIANG Kai YANG Qiang LI Long-Yun QIAO Chuan-Min GONG Huan- Fa REN Jun XING Yu-Yunl(State Key Laboratory for Pig Genetic Improvement and Production Technology, Jiangxi Agricultural University, Nanchang 330045, China Jiangsu Agri-animal Husbandry Vocational College, Taizhou 225300, China)

机构地区：[1]江西农业大学猪遗传改良与养殖技术国家重点实验室,南昌330045 [2]江苏农牧科技职业学院,泰州225300

出　　处：《农业生物技术学报》2016年第12期1882-1889,共8页Journal of Agricultural Biotechnology

基　　金：国家科技重大专项(No.2016ZX08006-003);国家自然科学基金(No.31402058)

摘　　要：促卵泡激素(follicle-stimulating hormone,FSH)是一种动物垂体前叶嗜碱性细胞合成分泌的糖蛋白类促性腺激素。FSH在雄性动物体内的主要作用是刺激精子发生。本研究以15 d和成年F1代转二花脸FSH公猪(Sus scrofa)及其同日龄全/半同胞野生型公猪睾丸为材料,分析了其绝对重量和相对重量,制作了睾丸苏木精-伊红(HE)染色石蜡切片,统计了相关指标,通过q RT-PCR分析了生殖相关基因在睾丸中的表达量,与此同时,检测了睾丸中FSHβ和FSHR的甲基化率。睾丸绝对重量和睾丸脏体比分析结果表明,二花脸FSH的高量表达对大白公猪睾丸重量没有显著影响。睾丸HE染色石蜡切片分析结果显示,二花脸FSH的高量表达可以增加大白公猪睾丸中生殖母细胞的数量,对大白公猪睾丸组织结构无显著影响。q RT-PCR分析结果表明,二花脸FSH的高量表达没有影响內源生殖相关基因的表达。甲基化水平检测结果发现,二花脸FSH基因的高量表达对大白公猪睾丸中FSHβ和FSHR的甲基化水平没有显著影响。本研究结果表明,二花脸FSH的高量表达可以增加大白公猪睾丸中生殖母细胞的数量,从而提高大白公猪的生精能力,为研究精子发生过程提供参考依据。Follicle-stimulating hormone（FSH） is a glycoprotein and gonadotropin hormone synthesized and secreted by animal anterior pituitary basophils. In males, FSH participates in spermatogenesis. In this study,weight analysis, histopathology analysis, q RT- PCR analysis and methylation analysis were performed to evaluate the influence of Erhualian FSH high- level expression on the testes of F1 Erhualian FSH transgenic（TG） boars and their wild- type（WT） male full/half sibs at the age of 15 d（n=6） and adulthood（n=10）. Six suckling piglets including 3 TG and 3 full-sib WT individuals and 10 adult boars including 5 TG and 5 halfsib WT individuals were slaughtered. Absolute weight and relative weight analysis of the testes were performed. There were no significant differences in the testes absolute weight of suckling piglets（P〈0.05）and adult boars（P〈0.05）. There were also no significant differences in the testes/body weight ratio of suckling piglets（P〈0.05） and adult boars（P〈0.05）. Testes absolute weight and testes/body weight ratio analysis showed that Erhualian FSH high- level expression had no influence on the testes weight. Paraffin sections of the testes samples were stained with hematoxylin- eosin（HE） and observed with a BA410microscope（Motic, China）. Gonocyte cells per unit area（about 171 100 μm2） for suckling piglets and intersitial cells per unit area（about 39 000 μm2） for adult boars were calculated. Area and perimeter per seminiferous tubule were also calculated using Image-Pro Plus 6.0 software. Only a significant increase were observed in gonocyte cells per unit area of 3 TG suckling piglets compared with that of 3 full-sib WT suckling piglets（P〈0.001）. Analysis of HE-stained paraffin sections of testes indicated that Erhualian FSH high-level expression could increase gonocyte number and had no effect on the organizational structure of the testes of Large White boars. The expression levels of genes related to reproduction in the testes were dete

关 键 词：促卵泡激素 转基因公猪 睾丸 

分 类 号：S814.8[农业科学—畜牧学]