PGC-1α基因在两个品种鸡两种表型肌肉中的差异表达  

Differential Expression Profile of PGC-1α Gene in Two Phenotypically Distinct Muscles Between Two Chicken(Gallus gallus) Breeds

作　　者：单艳菊[1] 束婧婷[1] 章明[1] 姬改革[1] 屠云洁[1] 张迪[1] 盛中伟[1] 范建华[1] 邹剑敏[1] SHAN Yan-Ju SHU Jing-Ting ZHANG Ming JI Gai-Ge TU Yun-Jie ZHANG Di SHENG Zhong- Wei FAN Jian-Hua ZOU Jian-Min(Key Laboratory of Poultry Heredity ＆ Breeding, Jiangsu Institute of Poultry Sciences, Yangzhou 225125, China)

机构地区：[1]江苏省家禽科学研究所家禽遗传育种重点实验室,扬州225125

出　　处：《农业生物技术学报》2016年第12期1900-1907,共8页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金(No.31301967和No.31572358);江苏省自然科学基金(No.BK20161322和No.BK20151317);现代农业产业技术体系建设专项资金(No.CARS-42);江苏省农业科技自主创新资金(No.CX(15)1009);江苏省重点研发计划(现代农业)(No.BE2015344);扬州市科技计划项目(No.YZ2014144)

摘　　要：过氧化物酶体增殖物激活受体γ辅激活因子1(peroxisome proliferator-activated receptor-γcoactivator-1 alpha,PGC-1α)是调控肌纤维类型转换的关键因子,已成为研究畜禽肌肉品质的一个热点基因。为探讨生长速度不同的两个品种鸡(Gallus gallus)生长发育早期两种表型肌肉中PGC-1α的表达情况,本研究利用q RT-PCR和Western blot分别检测PGC-1αmRNA和蛋白在慢生型清远麻鸡和速生型隐性白羽鸡的比目鱼肌(主要含慢肌纤维)和趾长伸肌(主要含快肌纤维)中的发育性(0,1,3,5,7和9周龄)表达变化。结果显示,同一种肌肉组织中PGC-1αmRNA均是0周龄时表达水平最高,显著高于其他各周龄(P<0.05);肌肉组织中PGC-1αmRNA和蛋白表达趋势虽然均不相同,但其表达均与肌肉表型和品种相关,即相同周龄时,两个品种鸡的比目鱼肌中PGC-1αmRNA和蛋白表达均高于其趾长伸肌,两种表型肌肉中PGC-1αmRNA和蛋白表达均是清远麻鸡高于隐性白羽鸡,且在0周龄时,mRNA表达差异均极显著(P<0.01)。结果提示,鸡骨骼肌PGC-1α表达可能与慢肌纤维含量密切相关,并与鸡肉品质存在关联。研究结果有助于了解PGC-1α在鸡骨骼肌中的作用,并为改良鸡肉品质提供理论依据。Peroxisome proliferator- activated receptor- γ coactivator- 1 alpha（PGC- 1α） has been a hot studying spot gene in meat quality traits of livestock because of its prominent role in regulation of muscle fiber type switching. To study the expression profile of PGC- 1α gene in 2 phenotypically distinct muscles between 2 chicken breeds at early growing stage, q RT-PCR and Western blot were used to detect the mRNA and protein expression of PGC-1α gene at 0, 1, 3, 5, 7 and 9 weeks in soleus and extensor digitorum longus between Qingyuan Partridge chicken（slow- growing chicken breed） and Recessive White chicken（fastgrowing chicken breed）. The results showed that the expression of PGC-1α mRNA of the 2 muscles declined firstly, then increased following by decreasing, and finally increased again. The expression peak of PGC- 1α mRNA of all the muscles appeared at 0 week on which the expression levels were significantly higher than that at other weeks（P〈0.05）, and the lowest expression in soleus in 2 chicken breeds appeared on 3 week,and the lowest expression in extensor digitorum longus in 2 chicken breeds appeared on 7 week. While the expression of PGC- 1α protein of the 2 muscles firstly gradually increased from 0 week to 5 week, then declined from 5 week to 7 week, and increased again from 7 week to 9 week. The expression peak of PGC-1αprotein in extensor digitorum longus in Recessive White chicken appeared at 5 week. Although the expression trends of mRNA and protein of PGC- 1α gene of the 2 muscles were not identical during early postnatal growth, they were highly muscle phenotype and breed specific. In general, the expression levels of mRNA and protein of PGC-1α gene in soleus were higher than those in extensor digitorum longus of both chicken breeds at the same weeks of age, and the expression of PGC- 1α mRNA in 2 muscles was extremely significant difference at 0 week（P〈0.01）. The expression levels of mRNA and protein of PGC-1α in Qingyuan Partridge chicken were higher than those in

关 键 词：鸡 过氧化物酶体增殖物激活受体γ辅激活因子1(PGC-1α) 肌肉 MRNA 蛋白 差异表达 

分 类 号：S831.2[农业科学—畜牧学]