分化相关基因-1在血管损伤后新生内膜形成中的表达及临床意义  

Expression and clinical significance of differentiation related gene-1 in the formation of new intima after vascular injury

作　　者：徐涛[1] 金法[1] 李宁[1] 叶志鹏[1] 吴可人[1] 李志杰[2] XU Tao JIN Fa LINing YE Zhipeng WU Keren LI Zhijie(Department of Hepatobiliary Surgery, the First Affiliated Hospital of Zhejiang Chinese Medical University, Hanghzou 310005, Zhejiang, China Department of Endocrinology, the Second Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, Zhejiang, China)

机构地区：[1]浙江中医药大学附属第一医院肝胆外科 [2]温州医科大学附属第二医院内分泌科

出　　处：《中国临床药理学与治疗学》2016年第10期1108-1113,共6页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：浙江省自然科学基金资助(LY16H060013)

摘　　要：目的:探讨分化相关基因-1在大鼠颈动脉球囊损伤后血管新生内膜形成中的表达情况及作用。方法:24只SD大鼠随机分为实验组和对照组,各12只。实验组给予建立颈动脉球囊损伤模型,对照组不做任何处理。第14天后分别测定两组大鼠血清中分化相关基因-1(N-myc downstream regulated gene 1,NDRG-1)、血管内皮生长因子(vascular endothelial growth,VEGF)、内皮抑素(endostatin,ES)、肿瘤坏死因子(tumor necrosis factorα,TNF-α)、白细胞介素-1(interleukin-1,IL-1)和内膜变化情况。结果:实验组12只大鼠建模成功10只,成功率83.33%。建模前两组大鼠NDRG-1、VEGF、ES、TNF-α、IL-1检测结果差异无统计学意义(P>0.05);建模后14 d实验组NDRG-1、VEGF、ES、TNF-α、IL-1检测结果明显高于对照组,差异均有统计学意义(均P<0.05);NDRG-1的表达与VEGF的表达呈正相关,与ES、TNF-α、IL-1的表达呈负相关(r=0.724,-0.822,-0.776,-0.814,P<0.01);实验组内膜面积、内膜面积/中膜面积比等明显大于对照组,差异均有统计学意义(均P<0.05),NDRG-1的表达与内膜面积、内膜面积/中膜面积比呈负相关(r=-0.712,r=-0.776,P<0.01)。结论:NDRG-1与血管损伤后新生内膜形成关系密切,可能是新生内膜增生的重要参与者。AIM： To investigate the expression and role of differentiation related gene-1 in the formation of blood vessel after carotid artery balloon injury in rats. METHODS： 24 SD rats were randomly divided into the experimental group and the control group（ 12 each）. The carotid artery balloon injury model was established in the experimental group,and the control group received no treatment. NDRG-1,VEGF,ES,TNF-α,IL-1 of serum,and endometrial changes fourteen days later were detected.RESULTS： 12 rats were modeled successfully（ success rate 83. 33%） in the experimental group.There were no significant differences in NDRG-1,VEGF,ES,TNF-α and IL-1 in the two groups before modeling（ P 〉 0. 05）; NDRG-1,VEGF,ES,TNF-α,IL-1 test results were significantly higher than those in the control group（ P 〈 0. 05） after fourteen days treatment; NDRG-1 expression and VEGF expression were positively correlated with ES,TNF-α and IL-1 expression were negatively correlated（ P 〈 0. 01）. The intimal area,neointimal area /media area ratio in the experimental group were significantly greater than that of the control group（ P 〈0. 05）. NDRG-1 expression and intimal area,neointimal area / media area ratio showed a negative correlation（ r =- 0. 712. R =- 0. 776, P 〈0. 01）. CONCLUSION： NDRG-1 is closely related to the formation of intimal hyperplasia after vascular injury,which may be an important participant in the intimal hyperplasia.

关 键 词：分化相关基因-1 血管内皮生长因子 血管损伤 内膜形成 

分 类 号：R965.2[医药卫生—药理学]