吉林省新育成大豆品种SSR指纹图谱身份证的构建  

Establishment of SSR Fingerprint ID for New Soybean Varieties in Jilin

作　　者：陈亮[1] 郑宇宏[1] 范旭红[1] 孟凡凡[1] 孙星邈[1] 张云峰[1] 王明亮[1] 王曙明[1] CHEN Liang ZHENG Yu - hong FAN Xu - hong MENG Fan - fan SUN Xing - miao ZHANG Yun - feng WANG Ming-liang WANG Shu-ming(Soybean Research Institute, Jilin Academy of Agricultural Sciences/National Engineering Research Center for Soybean, Changchun 130033, China)

机构地区：[1]吉林省农业科学院大豆研究所/大豆国家工程研究中心,吉林长春130033

出　　处：《大豆科学》2016年第6期896-901,共6页Soybean Science

基　　金：国家现代农业产业技术体系建设专项(CARS-04-PS11);吉林省农业科技创新工程(C62150403);吉林省科技发展计划(20140309010NY;20150520121JH);吉林省人社厅博士后人才项目(2013-2015);中国农业科技东北创新中心博士后进站项目(2013-2015)

摘　　要：为快速鉴定和准确评价大豆新品种,以吉林省98个大豆品种为材料,从大豆20个连锁群上均匀分布的320对SSR标记引物中初筛出12对扩增稳定、条带清晰、多态性高的引物,为以最少引物组合有效区分品种,揭示参试材料间丰富的遗传多样性,又进一步依据每对引物多样性指数的高低,复筛出了由7个SSR标记构成的核心引物组合,并最终以此构建吉林省近年育成大豆品种的SSR指纹图谱身份证。结果表明:98个参试大豆品种都有唯一的指纹图谱身份证代码,说明所选用的7个SSR核心标记可靠且有效;此外,以SSR标记扩增的整体带型作为编码依据能够有效提高大豆品种指纹图谱身份证构建的准确性。本研究完善了大豆品种指纹图谱身份证技术体系,能够满足当前吉林省乃至全国大豆品种分子鉴定的需求。Currently a novel method should be developed for the rapid identification and accurate evaluation of new soybean varieties. In this paper, a total of 98 released soybean varieties in Jilin were studied with SSR markers. Twelve SSR markers that had clear, stable and reproducible electrophoretic maps were selected from 320 randomly selected SSR markers located on each chromosome of soybean. In order to distinguish effectively soybean varieties with minimal primer pairs and reveal rich genetic diversities between the test materials, 7 core SSR markers were selected from aforementioned 12 SSR markers according to diversity index. At last, the bands amplified by each of the 7 core SSR markers were coded, and then the codes were combined as a fingerprint ID for soybean varieties. The results showed that each tested soybean variety had unique SSR fingerprint ID, and 7 core SSR markers were reliable and efficient. In addition, using the whole braid-types amplified by each of the 7 core SSR markers coded as fingerprint ID for soybean varieties can reduce complexity effectively in recording polymorphie amplification profiles. This paper not only improve the establishment method of SSR fingerprint ID for new soybean varieties, but also meet the requirements of molecular identification for new soybean varieties in Jilin province and even the whole country.

关 键 词：大豆 SSR 指纹图谱身份证 

分 类 号：S565.1[农业科学—作物学]