一种从大豆胞囊线虫中快速提取DNA的方法  

A Method for Rapid Extraction of DNA from Soybean Cyst Nematode

作　　者：练云[1] 冯文强[1,2] 雷晨芳[1] 李金英[1] 武永康[1] 王庭峰[1] 陆莉莉[1] 卢为国[1] LIAN Yun FENGWen-qiang LEI Chen-fang LIJin-ying WUYong-kang WANG Ting-feng LULi-li LU Wei-guo(Institute of Industrial Crops, Zhengzhou National Subcenter for Soybean Improvement/Key Laboratory of Oil Crops in Huang-huaihai Plains, Ministry of Agriculture, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian 271018, China)

机构地区：[1]河南省农业科学院经济作物研究所/国家大豆改良中心郑州分中心/农业部黄淮海油料作物重点实验室,河南郑州450002 [2]山东农业大学生命科学学院/作物生物学国家重点实验室,山东泰安271018

出　　处：《大豆科学》2016年第6期1043-1046,共4页Soybean Science

基　　金：国家自然科学基金(31371652);河南省农业科学院科研发展专项资金(20137905);国家现代农业产业技术体系建设专项(CARS-004);河南省科技攻关项目(162102110141)

摘　　要：由于大豆胞囊线虫(soybean cyst nematode,SCN)特殊的生长环境,用细菌专用DNA试剂盒提取方法提取DNA的质量不理想,而且耗时长,本文介绍了一种快速提取线虫DNA的方法。通过比较细菌专用DNA试剂盒提取法、质粒试剂盒提取法和CTAB法,发现利用质粒提取试剂盒,改良提取步骤,可以快速的提取质量较好的线虫DNA,r DNAITS区域序列扩增可有效扩增出目的基因,该方法可以直接用于线虫基因获取试验。In this paper, we reported a method for rapid extraction of DNA from soybean cyst nematode（SCN）. It is difficult and time consuming to obtain high quality DNA by using bacterial genomic DNA extraction kit for the special growth environ- ment of SCN. Three methods for extraction of DNA were compared in this study, including Bacterial genomic DNA extraction kit, San Prep column plasmid DNA Kit and CTAB method. Results showed that DNA isolated by improved San Prep column plasmid DNA Kit was high quality with time saving. We verified the quality by amplification sequence effectively from rDNA- ITS region. The method could be used for DNA obtaining from cyst and the later molecular manipulations of genes cloning.

关 键 词：大豆 大豆胞囊线虫 DNA提取 生理小种 

分 类 号：S435.651[农业科学—农业昆虫与害虫防治]