猪囊尾蚴凋亡蛋白酶抑制剂基因的克隆与表达  

Cloning and expression of apoptosis protease inhibitor gene from Cysticercus cellulosae

作　　者：王秋霞[1,2] 苏文强[2] 关俊勇 郭爱疆[1] 骆学农[1] 李辉[1] 欧长波[2] 余燕[2] 姜金庆[2] 马金友[2] 刘兴友[2] 王松[2] 张艳红[2] 才学鹏[1] WANG Qiu-xia SU Wen-qiang GUAN Jun-yong GUO Ai-jiang LUO Xue-nong LI Hui OU Chang-bo YU Yan JIANG Jin-qing MA Jin-you LIU Xing-you WANG Song ZHANG Yan-hong CAI Xue-peng(State Key Laboratory of Veterinary Etiological Biology/Key Laboratory ofA nimal Parasitology of Gansu Province/Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences ,Lanzhou 730046, China College of Animal Science and Veterinary Medicine ,Henan Institute of Science and Technology,Xinxiang 453003, China)

机构地区：[1]中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室甘肃省动物寄生虫病重点实验室,甘肃兰州730046 [2]河南科技学院动物科技学院,河南新乡453003

出　　处：《中国兽医科学》2016年第11期1365-1369,共5页Chinese Veterinary Science

基　　金：家畜疫病病原生物学国家重点实验室开放基金课题(SKLVEB2014KFKT007);河南科技学院高层次人才科研项目(2013008)

摘　　要：为了探究凋亡蛋白酶抑制剂基因在猪囊尾蚴生活过程中的作用,利用RT-PCR技术克隆了猪囊尾蚴凋亡蛋白酶抑制剂基因,并将其克隆至原核表达载体p ET-30a中,转化大肠杆菌BL21(D E3),经IPTG诱导后,用SDS-PAGE进行初步分析,在53 ku处出现明显的蛋白条带,与预期结果一致。W estern-blot结果表明,重组融合蛋白能够与抗H is特异性抗体发生反应。凋亡蛋白酶抑制剂基因的成功表达为后续对其功能进行研究奠定了基础。To explore the function of apoptosis protease inhibitor gene during life cycle of Cys- ticercus cellulosae, the apoptosis protease inhibitor gene was cloned from C. cellulosae by RT-PCR. The cDNA fragment was subcloned into pET-30a vector and expressed in Escherichia coli BL21（DE3）. SDS-PAGE analysis showed that the molecular weight of expressed fusion protein was approximately 53 ku. Wes- tern-blot showed that the recombinant protein could be recognized by anti-His antibody. The study laid the foundation for the further study of the function of apoptosis protease inhibitor gene.

关 键 词：猪囊尾蚴 凋亡蛋白酶抑制剂基因 克隆 表达 

分 类 号：S852.734[农业科学—基础兽医学]