江苏部分地区山羊产气荚膜梭菌病病原的分离鉴定  

Isolation and identification of Clostridium perfringens from goats in part area of Jiangsu Province

作　　者：金铎[1,2] 林家琪[1,2] 董真[1,2] 李军朝[1,2] 顾敏[1,2] 焦库华[1,2] 王彦红[1,2] 张信军[1,2] 刘文博[1,2] JIN Duo LIN Jia-qi DONG Zhen LI Jun-zhao GU Min JIAO Ku-hua WANG Yan-hong ZHANG Xin-jun LIU Wen-bo(College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China Jiangsu Co-Innovation Center for Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China)

机构地区：[1]扬州大学兽医学院,江苏扬州225009 [2]江苏省动物重要疫病与人兽共患病防控协同创新中心,江苏扬州225009

出　　处：《中国兽医科学》2016年第11期1401-1405,共5页Chinese Veterinary Science

基　　金：江苏高校优势学科建设工程;江苏省大学生创新训练计划省级重点项目(201311117039Z);江苏高校品牌专业建设工程资助项目(PPZY2015B158)

摘　　要：2014年10月—2015年2月,扬州大学动物医院畜禽门诊接诊了11例来自于扬州、南通地区的山羊腹泻病例,经临床症状分析和问诊,初步怀疑为产气荚膜梭菌感染。通过剖检,取肠道、肝、肾进行恒温厌氧分离培养,培养后得到表面光滑、半透明、圆屋顶状的菌落,经纯化、革兰染色、镜检以及生化特性分析,结果为产气荚膜梭菌。采取药敏纸片法进行药敏试验,结果显示,其对氟苯尼考的敏感性较强。From October 2014 to February 2015,11 cases of goats with diarrhea from Yangzhou, Nantong were brought to Yangzhou university animal hospital. The cases were suspected to be infected by Clostridium perfringens based on the clinical signs and epidemiological data. The intestine tracts, livers and kidneys were collected for the isolation and cultured in anaerobic incubator at 37 ℃. Smooth,semitransparent,and dome-shaped colonies were obtained. After further purification,the experiments of gram staining and microscopic examination, biochemical characterization analysis were carried out. The results showed that all these isolated strains were Clostridium perfringens. Drug sensitive tests were performed using the KB paper method,the results showed that the isolated strains were sensitive to florfenicol.

关 键 词：产气荚膜梭菌 分离 鉴定 药敏试验 

分 类 号：S852.616.3[农业科学—基础兽医学]