藏猪源益生芽胞杆菌的筛选鉴定及部分益生特性的研究  

Screening,identification and probiotic properties of probiotic bacillus from Tibetan pigs

作　　者：郭政宏[1] 周彪[1] 张怡[1] 严亨秀[1] GUO Zheng-hong ZHOU Biao ZHANG Yi YAN Heng-xiu(College of Life Science and Technology,Southwest University for Nationalities, Chengdu 610041 ,China)

机构地区：[1]西南民族大学生命科学与技术学院,四川成都610041

出　　处：《中国兽医科学》2016年第11期1424-1431,共8页Chinese Veterinary Science

基　　金：四川省应用基础研究项目(2014JY0038);中央高校专项基金项目(13NZYBS13);四川省教改项目(兽医专业学位研究生教育实践基地建设)

摘　　要：从藏猪粪便中分离筛选出具有良好益生潜力的益生芽胞杆菌,为藏猪源益生菌的开发奠定基础。先将健康藏猪粪便滤液于90℃水浴处理10 m in以杀死样品中的非芽胞杆菌,涂布于培养基后挑取单菌落纯化并扩大培养,再进行拮抗试验筛选能抑制常见病原菌的芽孢杆菌。综合形态学、生理生化特征及16S r D N A序列分析对筛选菌株进行鉴定,通过菌株耐受性试验、产水解酶试验、抗生素敏感试验等对菌株生物学特性进行研究。结果显示,筛选到3株对常见病原菌具有一定抑制作用的芽胞杆菌,其中,解淀粉芽胞杆菌SW U N-TP23在不利环境中具有较强的稳定性,可代谢产生纤维素酶、淀粉酶和蛋白酶,菌体内不含抗常用兽用抗生素的耐药基因。结果表明,分离筛选到的解淀粉芽胞杆菌SWUN-TP23有良好的潜在益生能力,具有进一步开发研究的价值。Probiotic Bacillus with good potential,which were isolated and screened from Tibet pig feces,provide a basis for the development of probiotics of Tibet pig source. Firstly,the healthy Ti- betan pig feces filtrate adopted the 90℃water-curing treatment for 10 minutes to kill non-bacillus coli in samples,after being coated on culture media,the single colonies were picked to purify and ex- panded,and then screened Bacillus capable of inhibiting common pathogenic bacteria by antagonistic tests. Through morphological,physiological and biochemical characteristics and 16S rDNA sequence anal- ysis,the selected strains were identified. Through tolerance tests,enzyme production tests and an- tibiotic sensitivityitests,the biological characteristics of strains were studied to select the best strains. In result,through screening,3 strains of Bacillus have a certain inhibitory effect on the common pathogenic bacteria. Among them,Bacillus subtilis SWUN-TP23 has a strong stability in the adverse environment, lt has strong stability in adverse environment and can produce cellulase,amylase and pro- tease. BaciJlusamyloliquefaciens SWUN-TP23 does not contain drug resistance genes against commonly used veterinary antibiotics. In conclusion,Bacillus amyJoliquefaciens SWUN-TP23,through isolation and screening,has go^d potential probiotic ability and is worthy of further studying and developing.

关 键 词：藏猪 芽胞杆菌 筛选 鉴定 生物学特性 

分 类 号：S852.616[农业科学—基础兽医学]