高原甘加藏羊发情周期垂体FSH β和LH β mRNA表达差异的研究  

Expression of FSH β mRNA and LH β mRNA in the pituitary of estrous cycle of Ganjia Tibetan sheep

作　　者：史军红[1] 何玉琴[1] 葛文博[1] 华道才让 韩志磊[1] 王文芳[1] 朱雪雁 SHI Jun-hong HE Yu-qin GE Wen-bo Huadaocairang HAN Zhi-lei WANG Wen-fang ZHU Xue-yan(College of Life Sciences and Technology,Gansu Agricultural University,Lanzhou 730070 ,China Ganjia Township Animal Husbandry and Veterinary Workstation of X iahe County in Gannan Tibetan Autonomous Prefecture ,X iahe 747199 ,China)

机构地区：[1]甘肃农业大学生命科学技术学院,甘肃兰州730070 [2]甘肃省甘南藏族自治州夏河县甘加乡畜牧兽医工作站,甘肃夏河747199

出　　处：《中国兽医科学》2016年第11期1444-1449,共6页Chinese Veterinary Science

基　　金：国家自然科学基金项目(31360595)

摘　　要：为了研究高原甘加藏羊发情周期垂体组织中促卵泡素β亚基(FSHβ)和促黄体素β亚基(LHβ)基因在m RNA水平的表达规律,选取发情周期不同阶段的高原甘加藏羊24只,从其垂体组织中提取总RNA,采用实时荧光定量PCR(q RT-PCR)技术,以β-actin为内参基因,比较研究了高原甘加藏羊发情周期垂体组织中FSHβ和LHβ基因在m RNA水平的表达差异。结果显示,FSHβ和LHβ的m RNA在高原甘加藏羊发情周期不同阶段的垂体组织中均有表达。发情前期、发情期、发情后期和间情期的FSHβm RNA相对表达量分别为0.235 9、1.0、3.547 5和3.666 7,发情前期与发情期、发情后期和间情期差异极显著(P<0.01),发情期与发情后期和间情期差异显著(P<0.05),发情后期与间情期差异不显著(P>0.05);LHβm RNA相对表达量为0.025 9、1.0、16.109 1和0.563 8,发情前期与发情期、发情后期和间情期差异极显著(P<0.01),发情期与发情后期差异极显著(P<0.01)、与间情期差异不显著(P>0.05),发情后期与间情期差异极显著(P<0.01)。因此,甘加藏羊发情周期内垂体组织中FSHβ和LHβ在m RNA水平上的表达呈现一种动态差异性变化趋势。本研究结果在分子水平上为揭示藏羊生殖内分泌调控机制提供了科学数据。The objective of this experiment is to examine the mRNA levels of FSH β gene and LH β gene in pituitary ofestrous cycle in Ganjia Tibetan sheep. The total RNA of pituitaries were sampled from 24 sheep in dif- ferent stages of estrous cycle and real-time fluorescence quantitative PCR （qRT-PCR） method with β-actin as reference gene was utilized to study above different expressiveness. The results showed that both FSH β gene and LH β gene were expressed in the pituitaries of Ganjia Tibetan sheep during the whole estrous cycle. The relative expression of FSH β mRNA in proestrus, estrus, metestrus and diestrus was 0.235 9,1.0,3.547 5 and 3.666 7, respectively, The expression of FSH β mRNA in proestrus was very significantly different from that other stage of estrous cycle （P〈0.01）, and The expression of FSH β mRNA in oestrus was very significantly different from that in both metestrus and diestrus （P〈0.05）,and The expression of FSH β mRNA in metestrus and diestrus was nosignificant difference （P〉 0.05 ）.The relative expression of LH β mRNA in proestrus, estrus, metestrus and diestrus was 0.025 9,1.0,16.109 1 and 0.563 8, respectively,The LH β mRNA in proestrus was very significantly different from that other stage of estrous cycle （P〈0.01）, The LH β mRNA the oestrus was significantly different in that of metestrus （P〈 0.01 ）, but it was no significant difference in that of diestrus （P〉 0.05 ）. The LH β mRNA was ex- tremely significant between metestrus and diestrus （P〈0.01）.Therefore the relative expression of FSHfl gene and LH β gene in mRNA levels presented a dynamic variant trend in Ganjia Tibetan sheep.This experiments also pro- vided the scientific data in molecular levels to study reproductive endocrine regulation mechanism of Tibetan sheep.

关 键 词：高原甘加藏羊 垂体 促卵泡素βmRNA 促黄体素βmRNA 发情周期 实时荧光定量PCR 

分 类 号：S852.2[农业科学—基础兽医学]