糖皮质激素和茶碱对慢性阻塞性肺疾病患者外周血T细胞表达CXCR3的影响  

The effects of glacocorticoids and theophylline on CXCR3 expression of peripheral blood T cell in COPD patients

作　　者：王文君[1,2] 杨晓霞[3] 罗玲[2] 吴健[2] 罗少华[2] 林琦[2] 高兴林[1,2] 

机构地区：[1]南方医科大学,广州510515 [2]广东省人民医院呼吸科广东省医学科学院广东省老年医学研究所,广州510080 [3]长治医学院附属和平医院,长治046000

出　　处：《中华老年医学杂志》2016年第11期1196-1200,共5页Chinese Journal of Geriatrics

摘　　要：目的探讨糖皮质激素和茶碱对慢性阻塞性肺疾病（COPD）患者T细胞表达CXCR3的作用。方法选取2009年3月至2011年1月未行治疗的COPD稳定期门诊患者21例，男18例、女3例，年龄（66．4土4．2）岁，第1秒用力呼吸量（FEV1）占预计值百分比为（60．8±7．2）％。取外周血单个核细胞，分为4组处理，分别与5mg／L及15mg／L氨茶碱、10nmol／L及100nmol／L的布地奈德共培养48h，流式细胞仪检测CD8＋T及CD4＋T淋巴细胞表面CXCR3的表达，计算CD8＋CXCR3＋T细胞百分比（CD8＋CXCR3＋T细胞／CD8＋T细胞）、CD4＋CXCR3＋T细胞百分比（CD4＋CXCR3＋T细胞／CD4＋T细胞）及其相应的平均荧光强度（MFI）。结果5mg／L及15mg／L氨茶碱作用下CD8＋CXCR3＋T细胞百分比、CD4＋CXCR3＋T细胞百分比及CD8＋CXCR3＋T细胞MFI与空白对照组比较差异无统计学意义；CD4＋CXCR3＋T细胞MFI（83．40±15．20和79．11±18．81）与空白对照组98．90±17．45比较有明显降低（F=7．676，P=0．001），5mg／L氨茶碱组与15mg／L氨茶碱组比较差异无统计学意义（P〉0．05）。10nmol／L及100nmol／L布地奈德作用下CD8＋CXCR3＋T细胞百分比[（41．05±14．27）％和（34．85±11．63）％]、CD4＋CXCR3＋T细胞百分比[（29．96±8．33）％和（26．27±7．34）％]、CD8＋CXCR3＋T细胞MFI（65．56±15．43和62．22±16，49）及CD4＋CXCR3＋T细胞MFI（69．66±15．37和63．75±14．29）均较空白对照组[（58．64±18．95）％、（37．11±11．70）％、84．92±19．79、98．90±17．453降低（F=13．597、7．350、10．569、29．959，P=0．000、0．001、0．000、0．000）；两种不同浓度布地奈德组比较差异无统计学意义（均P〉0．05）。结论小剂量或常规剂量氨茶碱抗炎作用可能与CD8＋T细胞cxCR3识别趋化因子能力无关，但可使CD4＋T细胞识别趋化因子能力减弱；糖皮质激素布地奈德则使T细胞识别趋化因子能力�Objective To investigate the effects of glucocorticoid and theophylline on CXCR3 expression of peripheral blood T cell in chronic obstructive pulmonary disease（COPD） patients. Methods Twenty-one patients （18 males and 3 females, aged 66.42±4.20 years in average, FEV1/ prediction value of（60.8±7.2）% with stable COPD and no treatment were recruited from March 2009 to January 2011. Peripheral blood mononuclear cells were isolated by density gradient centrifugation and cultivated respectively with 5 mg/L and 15 mg/L aminophylline, 10 nmol/L and 100 nmol/L of budesonide for 48 hours. Flow cytometry was applied to evaluate the expression level of CXCR3 on CD4＋ and CD8＋ T cells surface, and to calculate the percentage of CD4＋ CXCR3＋ cells over total CD4＋ cells（CD4＋ CXCR3＋ T cells/CD4＋ T cells）and the percentage of CD8＋ CXCR3＋ T cells over lotal CD8＋ cells（CD8＋ CXCR3＋ T cells/CD8＋ T cells）, and to detect the mean fluorescence intensity（MFI） of CXCR3＋ on CD4＋ and CD8＋ cells from the different groups. Results There were no statistically significant differences between 5 mg/L or 15 mg/L aminophytline group and the control group in percentages of CD4＋ CXCR3＋ cells over the total CD4＋ cells, of CD8＋ CXCR3＋ ceils over the total CD8＋ cells,and the MFI of CXCR3 on CD8＋ cells（all P〉0.05）. However,MFI of CXCR3＋ on CD4＋ cells were markedly decreased in two aminophylline groups versus the control group（P〈0.01）. There was no significant difference between two aminophylline groups（P^0.05）. The 10 nmol/L and 100 nmol/L of budesonide treatment group showed the markedly decreased levels of percentages of CD4＋ CXCR3＋ ceils over the total CD4＋ cells,of CD8＋ CXCR3＋ cells over the total CD8＋ ceils and the MFI of CXCR3＋ on CD4＋ and CD8＋ cells, as compared with control group（F= 13. 597, 7. 350, 10. 569, 29.959, P = 0. 000,0. 001,0. 000,0. 000）. There was no statistically significant difference between two budesonide groups（P〉0,

关 键 词：肺疾病 慢性阻塞性 受体 CXCR3 氨茶碱 布地奈德 

分 类 号：R563.9[医药卫生—呼吸系统]