山西市场动物饲料中转基因成分的检测（英文）  

Detection of transgenic components in animal feeds on Shanxi markets

作　　者：袁建琴[1] 常泓[1] 赵江河[1] 唐中伟[1] 史宗勇[1] 王俊东[2] Jianqin Yuan Hong Chang Jianghe Zhao Zhongwei Tang Zongyong Shi Jundong Wang(College of Life Science, Shanxi Agricultural University, Taigu 030801, Shanxi, China College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, Shanxi, China)

机构地区：[1]山西农业大学生命科学学院,山西太谷030801 [2]山西农业大学动物科技学院,山西太谷030801

出　　处：《生物工程学报》2016年第11期1576-1589,共14页Chinese Journal of Biotechnology

基　　金：“十二五”国家科技支撑计划子课题(No.2012BAD12B06-2);山西省科技攻关项目(No.20140311025-3);山西省自然科学基金(No.2013011028-2);山西省高等学校教学改革项目(No.J2012026)资助~~

摘　　要：为了评估转基因玉米和大豆在山西动物饲料市场的占有率和标识情况,采用改良十六烷基三甲基溴化铵法(Hexadecyltrimethy ammonium bromide,CTAB)提取山西市场抽取的30份鸡和猪饲料,通过定性PCR打包筛查,对检测阳性结果打包饲料拆包并检测CaMV35S启动子、NOS终止子、玉米内标zSSIIb、大豆内标Lectin和Cry IA(b)基因。同时检测玉米和大豆转化体事件MON810和GTS40-3-2。结果表明,83.3%的饲料含有转基因成分。所抽取的玉米、大豆、猪饲料和鸡饲料转基因成分阳性率分别为6.67%、100%、93.3%和73.3%。实时荧光定量PCR检测结果与定性PCR一致。结果提示,鸡和猪饲料中转基因成分在山西市场的占有率较高。To assess the presence of genetically modified （GM） maize and soybean in a range of commercialized feed in Shanxi province of China in 2015, improved hexadecyltrimethy ammonium bromide （CTAB） method was used to extract DNA. The screening of packed feeds was carried out by qualitative PCR. Then positive feeds were unpacked and detected by the CaMV 35S promoter, NOS terminator, zSS11b, Lectin and CrylA （b） genes. The identified maize and soybean events were confirmed by event-specific MON810 and GTS40-3-2. Results showed that 83.3% of the feeds was tested positive for GMOs, in which positive rates of maize, soybean, pig and layer feeds were 6.67%, 100%, 93.3% and 73.3%, respectively. The results of real-time PCR were consistent with qualitative PCR. These results indicated that commercialized GM feed had a wide positive product scope in Shanxi province of China. Further studies are necessary to study effects of feeding livestock and poultry with feed containing GM ingredients on animals and their products.

关 键 词：商业化鸡饲料 商业化猪饲料 转基因玉米 转基因大豆 定性PCR 实时荧光定量PCR 转化体事件MON810和GTS40-3-2 

分 类 号：S816.17[农业科学—饲料科学]