当归挥发油结肠靶向微丸在大鼠消化道内的释放行为  

Release characteristics of colon-targeted pellets of the volatile oil of Radix Angelica Sinensis in rats

作　　者：张庆[1] 茹庆国 彭宇[1] 马书伟[1] 吴清[1] ZHANG Qing RU Qingguo PENG Yu MA Shuwei WU Qing(School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102)

机构地区：[1]北京中医药大学中药学院,北京100102

出　　处：《北京中医药大学学报》2016年第10期855-859,共5页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(No.81073059)

摘　　要：目的研究当归挥发油结肠靶向微丸在大鼠消化道内的释放行为。方法给大鼠灌胃当归挥发油结肠靶向微丸,不同时间点处死大鼠,收集大鼠消化道内容物,高效液相色谱法（HPLC法）测定内容物中当归挥发油的指标成分藁本内酯的含量,以藁本内酯作为指标评价结肠靶向微丸在大鼠消化道内的释放行为,评价制备微丸的结肠靶向性。结果藁本内酯在大鼠消化道内容物中的线性范围为1.268-507.2 mg/L;在大鼠给药后4 h,即可在盲肠和结肠部位检测到有药物释放,而且微丸在大鼠消化道内持续释时间超过16 h,说明当归挥发油结肠靶向微丸可以在大鼠结直肠部位维持长时间的释药。结论制备的当归挥发油结肠靶向微丸有较好的结肠靶向性,可以用于后期炎症相关结直肠癌的化学预防研究中。Objective To study the release characteristics of colon-targeted pellets of volatile oil of Radix Angelica Sinensis（ Danggui） in rats. Methods Rats were administrated intragastrically with the colontargeted pellets of the volatile oil of Radix Angelica Sinensis. At different time points,the rats were sacrificed and their gastrointestinal tracts were resected,in which the contents were collected. Ligustilide,as the index of volatile oli of Danggui to evaluate the release characteristics of the colon-pellets in digestive tracts,was determined using HPLC. Results The linear range of ligustilide in the digestive tract of rats was 1. 268 - 507. 2 mg / L. Four hours after administration,ligustilide was detected in cecum and colon.And 20 h later,ligustilide could still be detected. That meant the colon-targeted pellets could maintain the drug release for a long time in colorectal sites of rats. Conclusion The prepared Danggui volatile pellets can be used as the carrier for targeting on colon,which can be used in the study of the chemo-prevention of inflammation related to colorectal cancer at the late stage.

关 键 词：当归挥发油 结肠靶向 消化道 高效液相色谱 大鼠 

分 类 号：R283.1[医药卫生—中药学]