负载血管内皮生长因子的纳米石墨烯涂层螺钉内固定治疗犬股骨颈骨折  

Internal fixation of femoral neck fracture using screws coated with vascular endothelial growth fac- tor-loaded nanographene

作　　者：李朔[1] 张显 潘建锋[1] 袁恒峰 范文帅[1] 朱梁[1] 张璟[1] 王忆茗[1] 查孝龙 李忱[1] 阎作勤[1] 郭常安[1] 

机构地区：[1]复旦大学附属中山医院骨科,上海200032 [2]江苏省江阴市璜土医院骨科,214400

出　　处：《中华创伤骨科杂志》2016年第11期981-987,共7页Chinese Journal of Orthopaedic Trauma

基　　金：上海市科学技术委员会科研计划项目（124119a7500）

摘　　要：目的探讨负载血管内皮生长因子（VEGF）的纳米石墨烯涂层螺钉中VEGF的缓释性能和生物活性，以及涂层螺钉内固定治疗犬股骨颈骨折的效果。方法制备纳米石墨烯涂层螺钉，利用涂层的吸附功能负载和缓释VEGF。观察涂层螺钉的表面结构并检测其细胞毒性。应用酶联免疫吸附测定负载VEGF的纳米石墨烯涂层螺钉的缓释性能。取10只健康成年犬，采用自身对照，构建犬股骨颈骨折模型，实验侧置入负载VEGF的纳米石墨烯涂层螺钉，对照侧置入空白纳米石墨烯涂层螺钉。利用X线片、组织病理学染色对结果进行评估。结果扫描电镜显示螺钉表面成功涂覆纳米石墨烯缓释涂层，且该涂层没有增加螺钉的细胞毒性。涂层螺钉可实现VEGF持续9d的有效缓释，最高释放量为（9．51±0．23）ng。X线片示实验侧愈合情况优于对照侧。实验侧骨小梁面积百分比（50．8％±3．3％）较对照侧（43．7％±3．9％）大，骨小梁宽度[（146．8±19．5）μm]较对照侧[（129．0±10．2）μm]增宽，但差异均无统计学意义（P〉0．05）。实验侧新生骨小梁面积（17．3％±1．5％）较对照侧（8．3％±1．6％）显著增大，微血管密度计数[（113．4±12．5）个／高倍镜]较对照侧[（61．2±9．4）个／高倍镜]显著增多，差异均有统计学意义（P〈0．05）。结论纳米石墨烯涂层可在空心螺钉表面负载和缓释VEGF。负载VEGF的纳米石墨烯涂层螺钉具备一定的生物活性，可以增强股骨颈骨折内固定术后股骨头内血管的修复能力。Objective To evaluate the internal fixation screws coated with vascular endothelial growth factor （VEGF）-loaded nanographene in the treatment of femoral neck fracture. Methods Screws with nanographene coating were prepared by direct liquid-phase exfoliation of the graphite. After the surface characteristics were observed through scanning electron microscopy （SEM）, the nanographene coating was loaded with VEGF through physical adsorption and controlled release of the VEGF was examined by ELISA. Femoral neck fracture models were built in 10 healthy adult canines to examine both the angiogenic and os- teogenic properties of the screws coated with VEGF-loaded nanographene which were implanted at the exper- imental sides while the screws with mere nanographene coating at the contralateral sides as controls. X-ray and histopathologic evaluations were used to assess the fracture healing progress. Results SEM demonstrated that the screws were coated successfully with VEGF-loaded nanographene. The coating did not increase the cytotoxity of screws. The accumulative release of VEGF clearly increased during the entire testing period （9 days） with a maximum release of 9.51 ± 0. 23 ng. X-ray and histopathologic examination proved better frac- ture healing at the experimental sides than at the contralateral sides. The percentage of trabecular area at the experimental sides （50. 8% ± 3.3% ） was insignificantly larger than at the control sides （43.7% ± 3.9% ）, and the trabecular width at the experimental sides （146. 8 ± 19. 5 μm） was insignificantly greater than at the control sides （129. 0 ± 10.2 μm） （ P 〉 0. 05） . The area of new bone trabecula at the experimental sides （17.3% ± 1.5% ） was significantly larger than at the control sides （8. 3% ± 1.6% ）, and the microvessel density （MVD） at the experimental sides （113.4 ± 12. 5） was also significantly greater than at the control sides （61.2 ± 9.4） （ P 〈 0.05）. Conclusions The nanographene coating can load and

关 键 词：股骨颈骨折 骨折固定术 内 骨钉 纳米复合物 血管内皮生长因子 

分 类 号：R687.3[医药卫生—骨科学]