鼠衣原体肺部感染中自然杀伤细胞对白细胞介素-22产生及疾病进程的影响  

Effect of natural killer cells on the production of interleukin-22 and disease process during Chlamydia muridarum lung infection

作　　者：董晓婧[1] 李静[1] 赵蕾[2] 齐眉[1] 孙泽雨 王红[1] 赵蔚明[1] 

机构地区：[1]山东大学医学院医学微生物学教研室,山东济南250012 [2]山东大学齐鲁医院基础医学研究所,山东济南250012

出　　处：《山东大学学报（医学版）》2016年第11期19-23,共5页Journal of Shandong University:Health Sciences

基　　金：国家自然科学基金(81271853);国家自然科学基金青年基金(81501761)

摘　　要：目的探究小鼠衣原体(Chlamydia)肺部感染中自然杀伤(NK)细胞对白细胞介素-22(IL-22)产生及疾病进程的影响。方法 36只小鼠分为实验组和对照组,每组18只。实验组小鼠尾静脉注射anti-asialo GM1,以封闭体内NK细胞,同时鼻吸入含鼠衣原体(Chlamydia muridarum)的感染液;对照组小鼠尾静脉注射同型对照抗体,感染方法同实验组。每天记录小鼠体质量变化;分别于感染后6、12 d,检测小鼠肺部衣原体包涵体形成单位(IFU);实时荧光定量PCR、ELISA方法检测小鼠脾脏中IL-22的表达,细胞内细胞因子染色技术检测小鼠脾脏Th17细胞数量变化。结果与对照组相比,实验组小鼠肺部感染加重,体质量降低趋势加大、肺部IFU增加(P<0.05),脾脏中IL-22的mRNA和蛋白表达水平减少(P<0.05),且Th17细胞的百分比和绝对数量均降低(P<0.01)。结论 NK细胞封闭导致小鼠体内IL-22的表达降低、肺部感染加重,提示NK细胞与IL-22的表达存在相关性;NK细胞可通过促进IL-22的产生,从而增强机体抗衣原体感染的能力。Objective To evaluate the effect of natural killer（NK） cells on the production of interleukin-22（IL-22） and disease process in Chlamydia lung infection. Methods Thirty-six mice were divided into experimental group（n = 18）and control group（n = 18）. Mice in experimental group or control group were injected anti-asialo GM1 antibody or isotype respectively,and then infected with Chlamydia muridarum intranasally. Body weight changes were monitored daily. Mice were sacrificed at day 6 and day 12 after infection,and inclusion-forming units（IFU） of lungs were measured. IL-22 expression was determined by real-time PCR and ELISA. The changes of Th17 cells numbers were measured by flowcytometry analysis. Results Comparied with control group,mice in experimental group deepened the lung infection and body weight decrease trend,exhibited the incresed lung IFUs,reduced splenic IL-22 expressions in mRNA and protein levels（all P 〈 0. 05）,and decreased percentage and absolute numbers of Th17 cells in spleen（both P 〈 0. 01）. Conclusion NK cells depletion results in reduced IL-22 expression and severe lung disease. NK cells can promote the expression of IL-22,which contributes to the effective control of Chlamydial lung infection.

关 键 词：衣原体 自然杀伤细胞 白细胞介素-22 

分 类 号：R374.1[医药卫生—病原生物学]