炎性细胞模型中姜黄素对胆固醇逆转运蛋白ABCA1和ABCG1基因的影响  

Effects of curcumin on gene expressions of reverse cholesterol transport proteins ABCAlversus ABCG1 in the cell model of inflammation

作　　者：吴彧[1] 夏彦清[2] 伍玥[2] 孙琳[1] 邬成业[2] 娄桂予[2] 李雅欣[2] 丁松泽[2] 孙恺[2] 

机构地区：[1]河南省人民医院康馨心内科,河南郑州450003 [2]河南省人民医院中心实验室,河南郑州450003

出　　处：《山东大学学报（医学版）》2016年第11期24-26,50,共4页Journal of Shandong University:Health Sciences

基　　金：郑州市金水区科技攻关项目(金科[2012]23号);河南省医学科技攻关计划省部共建项目(201201005)

摘　　要：目的探讨炎性反应模型中,姜黄素对胆固醇逆转运蛋白基因表达的影响。方法将RAW264.7细胞分为6组:空白对照组,姜黄素10μmol/L组,姜黄素20μmol/L组,脂多糖(LPS)组,姜黄素10μmol/L+LPS组和姜黄素20μmol/L+LPS组。应用荧光定量PCR检测各组中RAW264.7细胞腺苷三磷酸结合盒转运体A1(ABCA1)、腺苷三磷酸结合盒转运体G1(ABCG1)和肿瘤坏死因子-α(TNF-α)基因表达情况。结果 LPS刺激可显著上调RAW264.7细胞ABCA1和TNF-α基因的表达(P<0.05),下调ABCG1基因的表达(P<0.05);姜黄素处理后可进一步上调ABCA1基因的表达(P<0.05),但是对ABCG1和TNF-α基因的表达差异无统计学意义(P>0.05)。结论在炎性反应条件下,姜黄素可进一步上调胆固醇逆转运关键基因ABCA1的表达,这可能是其抗动脉硬化的分子机制之一。Objective To explore the effects of curcumin on reverse cholesterol transport protein gene expression in the cell model of inflammation. Methods RAW264. 7 cells were divided into 6 groups： control group,10 μmol / L curcumin group,20 μmol / L curcumin group,lipopolysaccharide（LPS） group,10 μmol / L curcumin ＋ LPS group,and20 μmol / L curcumin ＋ LPS group. The levels of mRNA expressions of ATP-binding cassette transporter A1（ABCA1）,ATP-binding cassette transporter G1（ABCG1） and tumor necrosis factor-α（TNF-α） in each group were determined by Real-Time PCR. Results LPS induction significantly increased ABCA1 and TNF-α mRNA expressions,and decreased ABCG1 mRNA expression（all P 〈 0. 05）. Pre-treating the cell with curcumin and stimulating with LPS did not change the expressions of TNF-α and ABCG1（both P 〉 0. 05）,but significantly increased the ABCA1 mRNA expression（P 〈 0. 05）. Conclusion Curcumin significantly amplifies the expression of reverse cholesterol transport key genes ABCA1 under the condition of the inflammatory reaction,which may be one of the molecular mechanisms of anti-arteriosclerosis.

关 键 词：腺苷三磷酸结合盒转运体A1 脂多糖 姜黄素 动脉硬化 

分 类 号：R34[医药卫生—基础医学]