全基因组预测枯草芽孢杆菌BEST195的分泌蛋白  

Prediction for Secreted Proteins from Bacillus subtilis subsp. natto BEST195 Genome

作　　者：任文来[1] 韩长志[2] 刘通[1] 夏秀芹[1] 

机构地区：[1]廊坊市文安县农业局,河北廊坊065800 [2]西南林业大学云南省森林灾害预警与控制重点实验室,云南昆明650224

出　　处：《西南林业大学学报（自然科学）》2016年第6期106-111,共6页Journal of Southwest Forestry University：Natural Sciences

基　　金：国家自然科学基金项目(31560211)资助;云南省森林灾害预警与控制重点实验室开放基金项目(ZK150004)资助;云南省优势特色重点学科生物学一级学科建设项目(50097505)资助;云南省高校林下生物资源保护及利用科技创新团队(2014015)资助;云南省教育厅科学研究基金项目(2014Y330)资助

摘　　要：枯草芽孢杆菌BEST195作为纳豆菌的一种,是纳豆生产的重要菌株之一。利用Signal P、Prot Comp、TMHMM、Phobius、Lipo P、Tat P等预测程序对该菌中4 456条蛋白质序列进行分泌蛋白找寻,同时,对上述分泌蛋白的氨基酸分布、信号肽长度大小、信号肽切割位点等性质进行分析。结果表明:枯草芽孢杆菌含有分泌蛋白102个,其氨基酸长度、信号肽长度与植物病原菌不同;信号肽切割位点属于A-X-A类型,与其他已经报道的植物病原真菌、细菌以及卵菌中分泌蛋白信号肽切割位点一致。通过上述生物信息学分析方法有效地实现了枯草芽孢杆菌BEST195分泌蛋白的预测,分泌蛋白的信号肽切割位点具有物种保守型特点。Bacillus subtilis subsp. natto BEST195 is one of the important strains produced natto. To identify the secreted protein from B. subtilis and clearly it′s characteristic. 4456 protein sequences in B. subtilis was analyzed to find the secreted protein using the program including SignalP, ProtComp, TMHMM, Phobius, LipoP and TatP. Meanwhile, the distribution of amino acids, the length of signal peptide as well as the signal peptide cleavage site of secreted protein was analyzed. 102 secrete proteins were found in B. subtilis. And the length of amino acids and the signal peptide were different the plant pathogens. The signal peptide cleavage site belongs to AXA type, which was same as other plant pathogenic fungi, bacteria and oomycete. Through the above bioinformatics analysis, the predicted secreted proteins can effectively achieve in B. subtilis, and the type of signal peptide cleavage site have consistent with other secreted proteins from different species.

关 键 词：枯草芽孢杆菌 分泌蛋白 信号肽 预测 

分 类 号：S759.81[农业科学—森林经理学]