临床分离肠球菌生物膜的形成能力及毒力基因的相关性研究  

The ability of biofilm formation in clinical Enterococcus and their correlation with virulence factors

作　　者：张肖肖[1] 马传玲 刘海洋[1] 毕文姿 卢鸿[1] 周铁丽[1] Zhang Xiao-xiao Ma Chuan-ling Liu Hai-yang Bi Wen-zi Lu Hong Zhou Tie-li(Laboratory Medicine Center, the First Affiliated Hospital of Wenzhou Medical university, Wenzhou 325035 Tongde Hospital of Zhejiang Province, Hangzhou 310012 School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou 325035)

机构地区：[1]温州医科大学附属第一医院医学检验中心,温州325035 [2]浙江省省立同德医院,杭州310012 [3]温州医科大学检验医学院,生命科学学院,温州325035

出　　处：《中国抗生素杂志》2016年第11期869-873,共5页Chinese Journal of Antibiotics

基　　金：浙江省卫生高层次创新人才培养工程项目(浙卫发[2012]241号)

摘　　要：目的了解本院临床分离肠球菌的生物膜形成能力以及其与毒力基因的相关性。方法收集温州医科大学附属第一医院2013年6月至2014年9月临床分离的肠球菌348株,其中粪肠球菌156株,屎肠球菌192株。采用结晶紫染色法测定肠球菌的生物膜形成能力,PCR方法筛查4种常见的肠球菌毒力基因esp、gel E、agg和ace的存在情况,统计分析肠球菌生物膜形成能力与毒力基因的关系。结果 348株肠球菌毒力基因esp、gel E、agg和ace的检出率分别为80.5%、46.0%、46.6%和44.3%,除esp基因外(P=0.078),粪肠球菌中的其他3种毒力基因的携带率均显著高于屎肠球菌(P<0.01);肠球菌的生物膜形成阳性率为54.6%(190/348),其中粪肠球菌的生物膜形成阳性率为75.6%(118/156),屎肠球菌的生物膜形成阳性率为37.5%(72/192);粪肠球菌esp、gel E和agg基因的携带与其生物膜形成阳性率密切相关,而屎肠球菌中,只有esp基因的携带与其生物膜形成阳性率密切相关(P<0.01)。结论本院分离的肠球菌具有较高的毒力基因携带率和生物膜形成能力,肠球菌毒力基因esp、gel E和agg的携带与其生物膜形成密切相关。Objective To investigate the ability of biofilm formation in Enterococcus isolated from our hospital and their correlation with virulence genes. Methods A total of 348 clinical Enterococcus isolates （156 Enterococcus faecalis and 192 Enterococcus faecium） were collected at the First Affiliated Hospital of Wenzhou Medical University in China during Jun 2013 to Sep 2014. The ability of biofilm formation assay were tested by crystal violet staining method, PCR method was used for screening of 4 common Enterococcus virulence factor genes, and then the relationship between the ability of biofilm formation and virulence factor genes was analysed. Results The rates of virulence factor genes （esp, gelE, agg and ace） of 348 strains of Enterococcus were 80.5%, 46.6%, 46% and 44.3% respectively. Except for the esp gene （P=0.078）, the rate of other 3 virulence factor genes in Enterococcus faecalis were significantly higher than that in Enterococcusfaecium （P〈0.01）. The positive rate of biofilm formation of Enterococcus was 54.6% （190/348）, whereas the positive rate of biofilm formation in Enterococcusfaecalis was 75.6% （118/156）, and the positive rate of biofilm formation in Enterococcus faecium was 37.5%（72/192）. Enterococcus faeealis virulence factor genes esp, gelE and agg were all closely related to biofilm formation, and Enteroeoeeusfaecium virulence factor gene esp was closely related to biofilm formation （P〈0.01）. Conclusion The carrying rate of virulence genes and the ability of biofilm formation were high in Enterococcus isolated in our hospital. Enterocoeeus virulence factor genes esp, gelE and agg were all closely related to biofilm formation.

关 键 词：肠球菌 毒力基因 生物膜 

分 类 号：R978.1[医药卫生—药品] R378[医药卫生—药学]