炮制对王不留行中刺桐碱及黄酮苷类成分含量及溶出率的影响  

Influence of Processing on Contents and Dissolution Rates of Hypaphorine and Flavonoid Glycosides in Vaccariae Semen

作　　者：周国洪[1] 唐力英[1] 寇真真[1] 王婷[1] 郭日新 王祝举[1] 

机构地区：[1]中国中医科学院中药研究所,北京100700

出　　处：《中国实验方剂学杂志》2016年第22期18-21,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金面上项目(81673602)

摘　　要：目的：研究炮制对王不留行中刺桐碱及黄酮苷类成分含量的影响,探索炮制对王不留行主要化学成分及其溶出率的影响。方法：采用HPLC测定炮制前后王不留行中刺桐碱、王不留行黄酮苷、肥皂草苷、异牡荆素-2″-O-阿拉伯糖苷和王不留行黄酮苷H的含量及水煎溶出率,流动相乙腈（A）-水（B）梯度洗脱（0~12 min,13%A;12~18 min,17%A;18~30 min,20%A）,检测波长280 nm。结果：炮制前,这5个成分的质量分数分别为0.13%,0.70%,0.018%,0.051%,0.11%,炮制后则分别为0.12%,0.52%,0.016%,0.098%,0.091%;5个成分的水煎溶出率分别升高了7.7%,10.2%,10.8%,11.4%,17.8%。结论：炮制后异牡荆素-2″-O-阿拉伯糖苷含量有较大幅度上升,而王不留行黄酮苷含量有较大下降,其余成分含量有少量下降。炮制对王不留行中刺桐碱及黄酮苷类成分含量的影响存在一定差异,但炮制后这些成分的水煎溶出率均上升。Objective： To investigate the influence of processing on contents of hypaphorine and flavonoid glycosides in Vaccariae Semen. Method： Before and after processing, HPLC was employed to determined contents and dissolution rates of hypaphorine,vaccarin, saponarin, isovitexin-2″-O-arabinoside and vaccarin H in Vaccariae Semen with the mobile phase of acetonitrile-water for gradient elution and detection wavelength at 280 nm. Result： Before processing,their contents were 0. 13%,0. 70%,0. 018%,0. 051% and0. 11%; after processing,their contents were 0. 12%,0. 52%,0. 016%,0. 098% and 0. 091%,respectively.However,dissolution rates of these five ingredients were raised by 7. 7%, 10. 2%, 10. 8%, 11. 4% and17. 8%,respectively. Conclusion： Processing has different influence on contents of hypaphorine and flavonoid glycosides in Vaccariae Semen,but their dissolution rates raise.

关 键 词：王不留行 炮制 黄酮苷 刺桐碱 溶出率 水煎液 

分 类 号：R283.1[医药卫生—中药学] R284.1[医药卫生—中医学]