黄芩茎叶中的化学成分  

Chemical Constituents from Stems and Leaves of Scutellaria baicalensis

作　　者：王宏伟[1] 尹志峰[1] 李洪波[1] 袁晓旭[1] 杨明明[1] 赵桂琴[1] 

机构地区：[1]承德医学院中药研究所,河北省中药研究与开发重点实验室,河北省中医药抗痴呆重点研究室,河北承德067000

出　　处：《中国实验方剂学杂志》2016年第22期41-44,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家科技支撑计划项目(2011BAI07B04)

摘　　要：目的:探讨黄芩茎叶的抗菌活性物质基础。方法:60%乙醇冷浸提取,通过硅胶柱色谱,聚酰胺柱色谱,羟丙基葡聚糖凝胶(Sephadex LH-20)柱色谱及HPLC,重结晶等方法分离纯化,根据化合物的理化性质和波谱数据(1H-NMR,13CNMR,UV,MS)鉴定化学结构。结果:从黄芩茎叶60%乙醇提取物中,分离得到8个单体化合物,分别鉴定为:芹菜素-7-O-β-D-葡萄糖醛酸苷(1),5,4'-二羟基-7-甲氧基二氢黄酮(2),芫花素(3),千层纸素A-7-O-β-D-葡萄糖醛酸苷(4),7-甲氧基白杨素(5),白杨素(6),3',4',5,5',7-五甲氧基黄酮(7),红花素-7-O-β-D-葡萄糖醛酸苷(8)。结论:化合物3为首次从黄芩茎叶中分离得到,化合物2,5,7为首次从黄芩植物中分离得到。Objective： To study the active components for antimicrobial activity in stems and leaves of Scutellaria baicalensis. Method： The components were extracted by using 60% ethanol cold immersion method,isolated and purified by silica gel chromatography,polyamide chromatography,Sephadex LH-20 column and HPLC, recrystallization and other methods. Their chemical structures were identified on the basis of physicochemical properties and spectral analysis（1H-NMR,13C-NMR,UV and MS）. Result： Eight monomeric compounds were isolated from 60% ethanol extracts for stems and leaves of S. baicalensis,and their structures were identified as carthamidin-7-O-β-D-gluclronide（1）,5,4＇-dihydroxy-7-methoxyflavanone（2）,genkwanin（3）,oroxylin-A-7-O-β-D-gluclronide（4）, 7-methoxychrysin（5）, chrysin（6）, 3＇, 4＇, 5, 5＇, 7-pentamethoxyflavone（7）,and carthamidin-7-O-β-D-gluclronide（8）. Conclusion： Compound 3 was isolated from stems and leaves of the plant for the first time. Compounds 2,5,7 were isolated from this plant for the first time.

关 键 词：黄芩茎叶 化学成分 分离纯化 结构鉴定 

分 类 号：R284.1[医药卫生—中药学] R284.2[医药卫生—中医学]