美洲大蠊水提取物对小鼠烫伤模型的影响  

Effect of Water Extract of Periplaneta americana on Scald Model in Mice

作　　者：杨胜群[1] 熊茜[1] 邹俊波[1] 张彤[1] 桑文涛 曾南[1] 

机构地区：[1]成都中医药大学药学院,中药材标准化教育部重点实验室,四川省中药资源系统研究与开发利用重点实验室-省部共建国家重点实验室培育基地,成都611137

出　　处：《中国实验方剂学杂志》2016年第22期145-149,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家基础科学人才培养基金项目(J1310034-09);四川省科技厅中药药理四川省青年科技创新研究团队项目(2014TD0007)

摘　　要：目的:观察美洲大蠊水提取物对小鼠实验性烫伤模型创面愈合的影响,并初步探讨其作用机制。方法:取健康ICR雌性小鼠,按体重分层随机分为正常组、模型组、康复新液组、美洲大蠊水提取物低、中、高(0.327,0.490,0.653 g·kg-1)剂量组,采用(82±2)℃砝码烫伤小鼠背部脱毛区皮肤制备模型,观察药物外用5,10 d对烫伤创面愈合的影响,并测定血清转化生长因子-β1(TGF-β1),白细胞介素-6(IL-6)含量,进行烫伤创面皮肤组织病理检查。结果:与模型组比较,美洲大蠊水提取物各剂量用药5,10 d均显著提高烫伤小鼠创面愈合率(P<0.05,P<0.01);中剂量组用药5 d明显提高小鼠血清TGF-β1水平,用药10 d则降低TGF-β1,IL-6含量,低剂量用药10 d亦降低TGF-β1含量(P<0.05)。美洲大蠊水提取物能促进烫伤小鼠皮肤纤维母细胞、成纤维细胞及毛细血管等增生,并能促进表皮、真皮及肌层损伤和真皮胶原纤维束排列紊乱的修复,尤以中剂量的修复作用明显。结论:美洲大蠊水提取物外用能明显促进烫伤模型小鼠创面的愈合,促进损伤表皮、真皮等的修复,作用机制可能与调控TGF-β1释放(早期促进,后期抑制)、抑制炎性因子释放有关,发挥既可促进修复但又避免瘢痕形成的作用。Objective： To observe the effects of the water extract of Periplaneta americana on the wound healing of experimental scald model mice,and explore its mechanism. Method： Healthy female ICR mice were randomly divided into control group,model group,Kangfuxin group,P. americana water extract low-dose group（0. 327 g·kg- 1）,middle-dose group（0. 490 g·kg- 1） and high-dose group（0. 653 g·kg- 1） group according to body weight,n = 13 in each group. The scald mouse models were established by using（82 ± 2） ℃ weight burning in hair removal zone on their back. The effect of the topical medicines（0. 1 m L / spray） on the scald wound was observed at 5 d and 10 d; the contents of transforming growth factor（TGF）-β1and interleukin（IL）-6 in serum were detected,and histopathological examination of burn wound skin was conducted. Result： As compared with the normal group,the wound healing rate was significantly reduced at day 5 and day 10 in model group（P 〈0. 01）,the level of IL-6 was significantly increased at day 10 in model group（P 〈0. 05）,with obvious pathological injury in skin. As compared with the model group,he wound healing rate was significantly increased at day 5 and day 10 in P. americana water extract groups（P 〈0. 05,P〈 0. 01）; the level of TGF-β1was significantly increased at day5 in P. americana water extract middle dose group（P 〈0. 05）; the levels of TGF-β1and IL-6 were reduced at day10（P 〈0. 05）; TGF-β1level was also reduced at day 10 in P. americana water extract low-dose group（P〈 0. 05）. Pathological test results showed that P. americana water extract can promote proliferation of skin fibroblasts,fibroblast,and capillary vessels in skin of scalded mice,and promote the repair of epidermis,dermis and muscular damages,as well as disorder of dermal collagen fibers,especially in the P. americana water extract middle-dose group. Conclusion： P. americana water extract has obvious promoting effect on would healing in scald mice,promoting repa

关 键 词：美洲大蠊水提取物 小鼠烧烫伤 创面愈合率 转化生长因子-β1 白细胞介素-6 

分 类 号：R285.5[医药卫生—中药学]