白芍-甘草药对治疗骨关节炎的疗效机制预测  

Prediction of Therapeutic Mechanism of Paeoniae Radix Alba-Glycyrrhizae Radix et Rhizoma Herbal Pair in Treating Osteoarthritis

作　　者：程仕萍[1,2] 周平生[3] 赵宁[2] 吕诚[2] 吕爱平[2,4] 谭勇[2] 

机构地区：[1]上海中医药大学,上海201203 [2]中国中医科学院中医临床基础医学研究所,北京100700 [3]江西中医药大学国际教育学院,南昌330006 [4]香港浸会大学中医药学院,香港999077

出　　处：《中国实验方剂学杂志》2016年第22期180-185,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金面上项目(81573845);国家自然科学基金青年项目(81403209);中国中医科学院自主选题项目(Z0412);北京市优秀博士学位论文指导教师科技项目(20118450201)

摘　　要：目的:探讨白芍-甘草药对治疗骨关节炎的作用机制。方法:首先在《中药大辞典(第二版)》中检索白芍和甘草的所有化学成分;其次采用中药系统药理学数据库(TCMSP)中的ADME参数,遴选活性成分;然后在Pub Chem和TCMSP数据库中查找活性成分对应的人类靶蛋白,在Gene数据库中查找骨关节炎相关基因;把靶蛋白和基因导入分子网络分析平台,采用核心计算分析法则,分别构建药物和疾病的分子网络和生物学通路,并进行比较映射分析。结果:药物和疾病涉及的共同生物学通路主要包括细胞免疫、细胞因子信号,涉及的共同高连接分子主要包括白细胞介素-1(interleukin-1,IL-1),肿瘤坏死因子-α(TNF-α),核转录因子-κB1(NF-κB1)和细胞外调节蛋白激酶(ERK1/2)。结论:白芍-甘草药对可能通过影响IL-1,TNF-α,NF-κB1和ERK1/2,以及调控细胞因子信号通路,抑制炎症,调节软骨细胞免疫,从而对骨关节炎产生协同疗效。Objective： To discuss the therapeutic mechanism of Paeoniae Radix Alba-Glycyrrhizae Radix et Rhizoma herbal pair（PG） in treating osteoarthritis. Method： Firstly,all of chemical components of peonyand licorice were retrieved in The Dictionary of Chinese Herbal Medicine（second edition）. Secondly,ADME parameters in the traditional Chinese medicine database system pharmacology（TCMSP） were applied in selecting active ingredients; then,human target protein corresponding to active ingredients were searched in Pub Chem and TCMSP databases,and osteoarthritis-related genes were searched in Gene database; target proteins and genes were introduced in the molecular network analysis platform,the core analysis method was used to build molecular networks and biological pathways for drugs and diseases,and compare the map analysis. Result： The common biological pathways involved by the drugs and diseases included cell immunity and cell factors,and the common high-connection elements they involved mainly included interleukin 1（IL-1）,tumor necrosis factor-α（TNF-α）,nuclear factor kappa B1 predominate（NF-κB1） and extracellular regulating protein kinase（ERK1 /2）.Conclusion： PG may have a curative effect on osteoarthritis by impacting IL-1,TNF-α,NF-κB1 and ERK1 /2,regulating cytokine signaling pathways,inhibiting inflammation and adjusting immune regulating cartilage cells.

关 键 词：白芍-甘草药对 骨关节炎 分子网络分析 疗效机制 

分 类 号：R285.5[医药卫生—中药学]