乙型肝炎病毒特异性CTL表位变异分析  

Analysis of variation in CTL epitope of Hepatitis B Virus

作　　者：邹淑慧[1] 肖九长[1] 张丽琴[1] 唐金凤[1] 刘奕红 

机构地区：[1]赣州市人民医院检验科,江西赣州341000

出　　处：《实验与检验医学》2016年第5期591-594,607,共5页Experimental and Laboratory Medicine

摘　　要：目的初步调查乙型肝炎病毒(HBV)特异性CTL表位的序列特点,同时探讨乙型肝炎重症化和慢性化的可能机理。方法本研究采用PCR扩增法获取HBV全基因并测序,参考国外文献报道的17个热点HBV特异性CTL表位氨基酸序列,分析其与本文获得的CTL表位氨基酸序列的差别。对患者各个表位变异的差异进行卡方检验。结果获得46份HBV全基因组序列,急性乙型肝炎(AHB)15例,慢性乙型肝炎(CHB)18例,慢性重型乙型肝炎(CSHB)13例。其中40份属于B基因型,6份属于C基因型。对17个表位进行分析发现,B基因型中变异较大的表位有S183-191,S382-390,C18-27,X36-44,X52-60和X115-123;C基因型中有P816-824,C18-27,X36-44,X52-60和X115-123。P816-824表位在CHB和CSHB组的变异率为27.8%和46.2%,显著高于AHB组(0%,P<0.01);C23-31表位在CHB组的变异率为22.2%,显著高于AHB和CSHB组(0%,0%,P<0.05)。结论了解HBV CTL表位序列特点,分析其与文献报道的CTL表位的差异情况,对防治乙型肝炎的重症化和慢性化具有指导意义。Objective To investigate the characteristics of CTL epitope of hepatitis B virus and explore the possible mechanisms of severity and chronicity of hepatitis B. Methods Serum samples were collected from patients infected with hepatitis B virus（HBV）,PCR method was used to amplify the whole gene of HBV DNA and the PCR products were sent to be sequenced directly. Amino acid sequences of 17 cytotoxic T Lymphocyte（CTL） epitopes previously reported abroad were taken as reference sequences,and comparison was made between these reference sequences and CTL epitopic sequences obtained from patients with chronic hepatitis B. The difference was calculated by χ~2 test. Result Complete HBV genome of 46 samples was successfully amplified and sequenced. 15 patients with acute（AHB）,18 chronic（CHB） and 13 chronic severe hepatitis B（CSHB）. 40 of which belongs to genotype B,while 6 belongs to genotype C. In the 17 analyzed CTL epitopes,S183-19,S382-390,C18-27,X36-44,X52-60 and X115-123 varies greatly in genotype B,while P816-824,C18-27,X36-44,X52-60 and X115-123 in genotype C. The mutation rates at P816-824 were higher in patients with CHB（27.8%） and CSHB（46.2%） than that in AHB（0%,P〈0.01）,C23-31 was also higher in patients with CHB（22.2%） than that in AHB and CSHB（0%,0%,P〈0.01）. Conclusion To investigate the characteristics of hepatitis B virus CTL epitopes in infected people and analyze the mutations will aid in treatment and prevention of severity and chronicity of hepatitis B.

关 键 词：乙型肝炎病毒 CTL表位 变异 

分 类 号：R446.62[医药卫生—诊断学] R512.62[医药卫生—临床医学]