调节性T细胞在青年与老年原发性高血压患者中的表达及临床意义  

Expression of Regulatory T Cells in Young and Elderly Patients with Essential Hypertension and the Clinical Significance

作　　者：吕彩霞[1,2] 王金丽[2] 杨艺[2] 阮磊[2] 操明[2] 张存泰[2] 

机构地区：[1]河北省人民医院老年病科,石家庄050051 [2]华中科技大学同济医学院附属同济医院综合科,武汉430030

出　　处：《华中科技大学学报（医学版）》2016年第5期555-558,共4页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：河北省自然科学基金青年基金资助项目(No.H2016307015)

摘　　要：目的探讨青年与老年原发性高血压(EH)患者CD4^+CD25^(high)FoxP3^+调节性T细胞(CD4^+CD25^(high)FoxP3^+Treg)的表达水平及炎症因子水平的差异。方法 50例EH患者与30例健康志愿者依据年龄分为:青年EH组(<50岁)、青年健康对照组、老年EH组(>60岁)和老年健康对照组。应用流式细胞术检测外周血CD4+CD25highFoxP3+Treg的表达,ELISA检测致炎细胞因子IFN-γ,及Treg相关抗炎细胞因子IL-10和TGF-β_1的表达。结果青年EH组CD4^+CD25^(high)FoxP3^+Treg和IL-10水平较青年对照组明显降低(均P<0.05),而老年组未见明显差异;青年和老年EH组IFN-γ水平较对照组均明显升高(均P<0.05)。结论 CD4^+CD25^(high)FoxP3^+Treg在EH中可能发挥调控炎症反应的作用,其在青年EH患者中的临床意义可能较在老年EH患者中更大。Objective To examine the expression of regulatory T cell（Treg）and the levels of inflammatory factors in young and elderly patients with essential hypertension（EH）.Methods The percentages of circulating CD4^＋CD25^（high） FoxP3^＋ Treg were evaluated by flow cytometry and the levels of IL-10,TGF-β1and IFN-γ by ELISA among young EH patients（50years,n=25）,young controls（n=15）,elderly EH patients（〉60years,n=25）and elderly controls（n=15）.Results Young EH patients had lower percentages of CD4^＋CD25^high FoxP3^＋ Treg and lower levels of IL-10 than the young controls（both P〈0.05）,but there was no significant difference in the percentages of CD4^＋CD25^high FoxP3^＋ Treg and the levels of IL-10 between the elderly patients and their controls（both P〉0.05）.The levels of IFN-γwere significantly increased in young and elderly patients as compared with their age-matched controls（both P〈0.05）.Conclusion CD4^＋CD25^high FoxP3^＋ Treg cells may regulate the inflammatory responses in EH.They play a more important role in young patients than in elderly patients with EH.

关 键 词：原发性高血压 调节性T细胞 炎症反应 

分 类 号：R544.11[医药卫生—心血管疾病]