灯盏花乙素对肝星状细胞及TGF-β1/Smad信号通路的作用评价  

Effect of scutellarin on TGF-β1/Smad signaling pathway in hepatic stellate cell

作　　者：闫瑢玓[1] 方慧[2] 迟莉[2] 

机构地区：[1]山东中医药大学第二附属医院职业病科,济南250001 [2]首都医科大学中医药学院中医基础学系

出　　处：《环球中医药》2016年第11期1314-1317,共4页Global Traditional Chinese Medicine

基　　金：2015年首都中医药研究专项(15ZM08)

摘　　要：目的观察灯盏花乙素(scutellarin)对肝星状细胞(hepatic stellate cell,HSC)及转化生长因子β1(TGF-β1)/Smad信号通路的影响,探讨scutellarin抗肝纤维化的机制。方法建立TGF-β1诱导的HSC LX-2模型,CCK-8法检测scutellarin对细胞增殖的作用,化学发光法检测scutellarin对Caspase3/7活性的影响,酶联免疫吸附测定(enzyme linked immunosorbent assay,ELISA)法检测细胞上清液Ⅰ型前胶原的含量,ELISA法检测细胞Smad2和Smad3磷酸化水平。结果scutellarin能抑制HSC增殖,增强细胞Caspase3/7活力,抑制Ⅰ型前胶原分泌,降低LX-2细胞Smad2和Smad3磷酸化水平。结论抑制TGF-β1/Smad信号通路可能是scutellarin抗肝纤维化的作用机制。Objective To observe the effect of scutellarin on signaling pathway in hepatic stellate cells(HSC)and transforming growth factor beta 1(TGF-beta 1),to explore the mechanisms of antihepatic fibrosis.Methods Experiments were conducted by human HSC cells line.Transforming growth factor-β1(TGF-β1)was used to induce HSC model.The inhibitory effect of scutellarin on LX-2 cells was observed.Chemiluminescence measurement was used to measure the activity of caspase3/7 in LX-2 cells.ELISA was used to detect the content of pro-collagen Ⅰ in LX-2 supernatant.The expressions of PhosphoSmad2 and Phospho-Smad3 were measured by ELISA.Results Scutellarin could Inhibit LX-2 cells proliferation,pro-collagen,I secretion,and phosphorylation of Smad2,Smad3 expressions.Scutellarin could improve the activities of caspase3/7.Conclusion The mechanism of scutellarin to anti hepatic fibrosis may be the inhibition of TGF- beta 1/Smad signaling pathway

关 键 词：灯盏花乙素 肝星状细胞 TGF-Β1/SMAD信号通路 

分 类 号：R619.6[医药卫生—外科学]