光慈菇组培种苗商品化生产技术应用试验  

Application Test on Commercial Production Technology of Bulus tulipae Tissue Culture Seedlings

作　　者：陈冬怡 陈国华[1] 胡群英[1] 黄春华[1] 黄芳[1] 叶秀粦[1] 朱恒昌[1] 

机构地区：[1]广东省应用植物学重点实验室中国科学院华南植物园,广州510650

出　　处：《农业工程》2016年第5期128-131,共4页AGRICULTURAL ENGINEERING

基　　金：2013年中央财政专项农业技术推广与服务补助资金项目"名贵中药材克隆及高效复合栽培技术体系的示范与推广"

摘　　要：以光慈菇的全鳞茎、心芽、茎尖和种子为外植体,利用植物组培快繁技术培养出完整的无毒植株。试验结果表明,外植体灭菌处理成功率为92%,继代增殖系数最高达2.6,生根率为91.3%,组培苗移栽成活率为95%。该研究成果为光慈菇产业的可持续发展提供了有力的技术支撑。Taking whole bulb,heart bud,shoot tip and seed of Bulus tulipae as explant,complete and virus-free plants were obtained by tissue culture technology. Test results showed that success rate of sterilization of explants was 92%,subculture multiplication factor was up to 2. 6,rooting rate was 91. 3% and transplanting survival rate of tissue culture seedlings was 95%.This research results provided a strong technical support for sustainable development of Bulus tulipae industry.

关 键 词：光慈菇 组培快繁技术 外植体 产业化 

分 类 号：S567.239[农业科学—中草药栽培]