何鲁牵牛的组培快繁研究  

Study on Tissue Culture and Rapid Propagation of Ipomoea holubii

作　　者：吕永平[1] 王燕[1] 牟豪杰[1] 汪一婷[1] 李海营[1] 

机构地区：[1]浙江省农业科学院病毒学与生物技术研究所,浙江杭州310021

出　　处：《安徽农业科学》2016年第30期110-111,199,共3页Journal of Anhui Agricultural Sciences

基　　金：国际先进农业科技术计划(948)项目(2011-G31);浙江省农业科学院青年人才培养项目(2015R21R08E08)

摘　　要：[目的]建立何鲁牵牛的组培快繁体系,为其规模化生产提供理论依据。[方法]以何鲁牵牛茎段为外植体,研究何鲁牵牛不定芽增殖和生根的最适培养基。[结果]何鲁牵牛在WPM+0.3 mg/L 6-BA+0.01 mg/L NAA+0.1 g/L活性炭的启动培养基上成功诱导腋芽抽出,将腋芽转接到WPM+0.3 mg/L 6-BA+0.1 g/L活性炭培养基上进行不定芽的增殖,增殖率达4.30;在WPM+0.1 mg/L IBA+0.1 mg/L NAA+0.1 g/L活性炭培养基上生根率较高,可达90%。[结论]该研究建立了何鲁牵牛的组培快繁体系,有利于何鲁牵牛种质资源保护和工厂化生产。[Objective] To establish tissue rapid propagation system of Ipomoea holubii,and to provide theoretical foundation for its large-scale production.[Method] Taking the stem of Ipomoea holubii as explants,we researched the optimal culture medium for adventitious buds prolifera-tion and rooting of I.holubii.[Result] Axillary buds were induced on WPM＋0.3 mg/L 6-BA ＋0.01 mg/L NAA ＋0.1 g/L active carbon （AC）.The shoots of I.holubii were cultured on WPM ＋0.3 mg/L 6-BA ＋1 g/L AC for multiplication,and the multiplication ratio was 4.30. WPM ＋0.1 mg/L IBA＋0.1 mg/L NAA＋0.1 g/L AC was the optimal medium for rooting,and the rooting rate was 90%.[Conclusion] This re-search establishes the tissue culture and rapid propagation system of I.holubii,which is helpful to the germplasm protection and industrial produc-tion of I.holubii.

关 键 词：何鲁牵牛 组培快繁 增殖 生根 

分 类 号：S604[农业科学—园艺学]