靶向肺癌干细胞的多肽Gd基T_1型MRI探针的研究  

A Gadolinium-Based T_1 MRI Probe for Detection of Lung Cancer Stem Cells

作　　者：张宏岩[1,2] 李春林[2] 英晓芳[2] 魏建东[1] 张海禄[2] 邓宗武[2] 朱毅敏[2] 谭波[2] ZHANG Hong-yan LI Chun-lin YING Xiao-fang WEI Jian-dong ZHANG Hai-lu DENG Zong-wu ZHU Yi-min TAN Bo(Institute of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, China Suzhou Institute of Nano-tech and Nano-bionics, Chinese Academy of Sciences, Suzhou 215123, China)

机构地区：[1]上海大学环境与化学工程学院,上海200444 [2]中国科学院苏州纳米技术与纳米仿生研究所,江苏苏州215123

出　　处：《波谱学杂志》2016年第4期627-634,共8页Chinese Journal of Magnetic Resonance

基　　金：国家自然科学基金资助项目(31371010)

摘　　要：用固相多肽合成技术制备了特异性靶向肺癌干细胞的T_1型多肽核磁共振成像(MRI)探针——Gd-DOTA-HCBP-1.在11.7 T静磁场条件下,其纵向弛豫效率(r1)为6.15 mmol^(-1)·L·s^(-1),是商用T1造影剂Dotarem的1.6倍.体外细胞成像表明,此探针的使用可显著提高肺癌干细胞克隆球的可观测性,单个克隆球(包含2 000~4 000个细胞)可被明显辨识.A T1 magnetic resonance imaging （MRI） contrast agent specific to lung cancer stem cells Gd-DOTA-HCBP-1 was synthesized by solid-phase peptide synthesis technology. Its longitudinal relaxation rate （r1） was 6.15 mmol^-1·L·s^-1, approximately 0.6 times higher than that of the commercial T1 contrast agents Dotarem at 11.7 T. In vitro MRI experiments indicated that the detectability of the cancer stem cells could be significantly improved to about 2 000-4 000 cells per sphere by the use of the probe.

关 键 词：磁共振成像(MRI) 多肽分子探针 肺癌干细胞 T1造影剂 

分 类 号：O482.53[理学—固体物理]