茯苓酸通过激活多聚腺苷二磷酸核糖聚合酶诱导人乳腺癌细胞MDA-MB-231凋亡  

Induction of pachymic acid on apoptosis of human breast cancer MDA-MB-231 cells by activating Poly ADP ribose polymerase

作　　者：江薇[1] 

机构地区：[1]江西中医药高等专科学校,江西抚州344000

出　　处：《中草药》2016年第21期3861-3865,共5页Chinese Traditional and Herbal Drugs

摘　　要：目的观察茯苓酸对人乳腺癌MDA-MB-231细胞增殖及凋亡的影响,并初步探讨作用机制。方法乳腺癌MDA-MB-231细胞与不同浓度(1、2、5μmol/L)的茯苓酸共孵育,采用CCK-8细胞增殖与活性检测试剂盒检测细胞存活率;流式细胞术Annexin V/PI双染色检测细胞凋亡;Western blotting法检测多聚腺苷二磷酸核糖聚合酶(PARP)及与细胞凋亡相关蛋白的表达。结果茯苓酸能够剂量和时间依赖性地抑制MDA-MB-231细胞的增殖,并可诱导MDA-MB-231细胞凋亡。浓度为1、2、5μmol/L的茯苓酸作用MDA-MB-231细胞48 h后,细胞凋亡率显著增加至25.6%、59.4%、87.2%,明显高于对照组凋亡率(5.4%);经过1、2、5μmol/L茯苓酸分别处理48 h后,实验组MDA-MB-231细胞中凋亡蛋白标志物PARP裂解量升高,且呈剂量依赖性。结论茯苓酸能抑制MDA-MB-231细胞的增殖并诱导其凋亡,其作用机制与激活PARP有关。Objective To study the potential apoptotic effect of pachymic acid isolated ~om Poria cocos, a traditional Chinese medicinal fungi, on human breast cancer cells. Methods Pachymic acid was isolated and purified from the crude ethanol extract of P. cocosl Human breast cancer cell line MDA-MB-231 cells were treated with pachymic acid. Relative cell viabilities were determined by the Cell Counting Kit-8 assay; Apoptosis was analyzed by Annexin V/PI dual staining with flow cytometry; The protein expression levels of poly ADP ribose polymerase （PARP） and other apoptotic related proteins were detected by Western blotting. Results Pachymic acid reduced the viability of MDA-MB-231 ceils dose- and time-dependently. Moreover, pachymic acid induced the apoptosis in MDA-MB-231 cells. The data of Western blotting showed that pachymic acid up-regulated the expression level of PARP, in a dose-dependent manner. In addition, apoptosis-related proteins like Bax, Bcl-2, and casapases were all affected by pachymic acid. Conclusion Pachymic acid could induce the apoptosis in human breast cancer cells, which provides a novel information for clinical application ofpaehymie acid and its preparations for the breast cancer patients.

关 键 词：茯苓酸:乳腺癌 MDA-MB-231 细胞 增殖 凋亡:多聚腺苷二磷酸核糖聚合酶 

分 类 号：R285.5[医药卫生—中药学]