TLR4激活剂对约氏疟原虫感染早期免疫应答的影响  

Effect of TLR4 against Early Immune Response during Plasmodium yoelii Infection

作　　者：吕衍民 郁春云 刘飞[1] 刘军[1] 曹雅明[1] 

机构地区：[1]中国医科大学基础医学院免疫学教研室,辽宁沈阳110122

出　　处：《微生物学杂志》2016年第5期57-61,共5页Journal of Microbiology

基　　金：2011年度高等学校博士学科点专项科研基金项目(20112104110018)

摘　　要：通过外源性给予LPS,探讨TLR4在疟疾感染早期免疫应答的作用特点及其免疫调节作用。通过Plasmodium yoelii 17XL感染的BALB/c小鼠建立鼠疟模型并在感染前给予LPS,于感染第0、3和5天制备脾细胞悬液,通过流式细胞术检测脾细胞悬液中TLR4+DCs和Tregs百分含量;ELISA方法检测脾细胞培养上清中IFN-γ和IL-10水平。结果显示,LPS处理能够显著延长宿主生存期,降低原虫血症水平,同时显著提升脾上清中的IFN-γ水平,降低抑炎性细胞因子IL-10水平。在感染早期,LPS处理可诱导Th1型免疫应答的有效建立,明显遏制P.y 17XL红内期疟原虫的感染进程。The effective characteristics and the role of TLR4 in early immune response and immune regulation were investigated during plasmodium infection by exogenous administration of LPS,Malaria model was established by Plasmodium yoelii 17 XL infected BALB / c mice and the LPS was administered prior to the infection. Spleen cell suspension was prepared on day of 0,3 and 5 after the infection. The percentages of TLR4＋DCs and Tregs were detected by flow cytometry. The concentration of IFN-γ and IL-10 were tested by Enzyme-Linked Immuno Sorbent Assay（ ELISA）. The results showed that LPS treatment could reduce the parasitaemia level and prolong the survival time. At the same time the IFN-γ levels in spleen supernatant were significantly increased and reduced the levels of inflammation-inhibitory cytokines. In the early stages of infection,the treatment of LPS could effectively establish Th1 immune response and significantly contain the P. y 17 XL infection process during endoerythrocytic stage.

关 键 词：TLR4 Th1型免疫应答 致死型约氏疟原虫 保护性免疫 

分 类 号：Q939.91[生物学—微生物学] R392[医药卫生—免疫学]