环氧树脂固定化卤醇脱卤酶的研究  

Immobilization of halohydrin dehalogenase by ES-103B epoxy resin

作　　者：顾恺[1] 邹树平[1] 王志才[1] 郑裕国[1] 

机构地区：[1]浙江工业大学生物工程研究所,浙江杭州310014

出　　处：《现代化工》2016年第11期69-72,74,共5页Modern Chemical Industry

基　　金：国家自然科学基金(21176224)

摘　　要：采用环氧树脂ES-103B对卤醇脱卤酶进行固定化,对最优固定化条件及固定化酶的操作稳定性进行了研究。结果最佳固定化条件为:吸附温度为25℃,吸附缓冲液为p H 7.0磷酸钠缓冲液,初始酶质量浓度为0.5 mg/m L,共价时间为24 h。在此优化条件下,固定化酶的酶活回收率为74.5%,酶蛋白固定化效率为94.2%,比活力为518.6 U/g。利用固定化酶催化1,3-二氯丙醇制备环氧氯丙烷,重复使用15批次后,产物收率仍能保持在初始收率的91%以上,具有良好的工业化应用潜力。The immobilization of halohydrin dehalogenase is performed by epoxy resin ES-103 B. The immobilization condition is optimized and the operation stability of immobilized enzyme is also investigated. The optimal immobilization conditions are shown as follows ： 25℃ of absorption temperature, sodium phosphate buffer as the immobilization buffer, 7.0 of pH ,0. 5 mg/mL of the initial concentration of enzyme and 24 h of alkaline treatment time. Under this condition, the recovery activity of enzyme and the immobilization yield reach 74. 5% and 94. 2% ,respectively. The specific activity is 518. 6 U/g. The immobilized enzyme can be successfully run for 15 batches and the yield still remains above 91% , which has a great potential for industrialization.

关 键 词：固定化 环氧树脂 卤醇脱卤酶 

分 类 号：Q814.2[生物学—生物工程]