穴位埋线联合东莨菪碱治疗对吗啡依赖大鼠十二指肠表达5-羟色胺的影响  

Effects of Acupoint Catgut Embedding Therapy Combined Scopolamine on the Levels of 5-Hydroxytryptamin of Duodenum in Morphine Dependent Rats

作　　者：刘霞[1] 郭俊峰[1] 蒋德梅[1] 徐照[2] 冯玲媚[3] 钱景丽 艾飞[4] 

机构地区：[1]贵阳中医学院组织学与胚胎学教研室,贵阳550002 [2]贵阳中医学院形态学实验室,贵阳550002 [3]贵阳中医学院针灸推拿学院,贵阳550002 [4]贵阳中医学院第二附属医院,贵阳550002

出　　处：《实验动物科学》2016年第5期43-47,51,共6页Laboratory Animal Science

基　　金：贵州省科技厅贵阳中医学院联合基金;黔科合中药字[2011]LKZ7040号

摘　　要：目的探讨穴位埋线加东莨菪碱治疗对吗啡依赖大鼠十二指肠5-羟色胺(5-HT)免疫反应(IR)细胞的影响。方法本试验将大鼠随机分为正常组、自然戒断组(简称戒断组)、东莨菪碱治疗组(简称西药组)、穴位埋线治疗组(简称穴位埋线组)及穴位埋线加东莨菪碱治疗组(简称穴位埋线加西药组)。腹腔注射吗啡建立吗啡依赖大鼠模型,经不同的方法干预后,观察戒断症状,并于干预后7d和14d分别取材。观察十二指肠5-HT-IR细胞形态特点,并比较各组十二指肠组织匀浆中5-HT的含量。结果吗啡依赖大鼠戒断症状明显,其十二指肠黏膜5-HT-IR细胞数和组织匀浆中5-HT含量均明显增加(P<0.01);治疗7d,与自然戒断组比较,各治疗组均有明显效果(P<0.05),并以穴位埋线加西药组改善最为显著(P<0.01),几乎恢复到正常水平;治疗时间延长至14d,除自然戒断组外,各治疗组的检测指标均接近正常组(P>0.05)。结论西药治疗与穴位埋线治疗对吗啡依赖所致大鼠的戒断症状及十二指肠分泌5-HT的功能均有逆转作用,两种方法结合可缩短治疗时间,治疗效果更佳。Objective To observe the effects of acupoint catgut embedding therapy combined western medicine scopolamine on the levels of 5-hydroxytryptamin（ 5-HT） of duodenum in morphine dependent rats. Method The rats were randomized into Control group,Spontaneous withdrawal group,Scopolamine group,Acupoint catgut embedding group,and Acupoint catgut embedding combined scopolamine group. Morphine dependent rat model was induced by administration（ ip） of morphine. Withdrawal syndromes were evaluated after different treatments.Rats were sacrificed after treated for 7 and 14 days respectively. Compare morphological changes,5-HT levels of duodenum among different groups. Result Withdrawal syndromes appeared in morphine dependent model group obviously with increased 5-HT expression in duodenum and tissue homogenate. All detecting items of Acupoint catgut embedding combined scopolamine group were improved significantly after treated for 7 days,there was no significant difference compared to Control group. Detecting items of Scopolamine group were close to the Control group after treated for 14 days（ P 〉 0. 05）. Conclusion Both western medicine scopolamine and acupoint catgut embedding therapy could reverse withdrawal syndromes,5-HT levels of duodenum in morphine dependent rats.However,combined two therapies could shorten the time of treatment with better effect.

关 键 词：穴位埋线 东莨胆碱 吗啡依赖 十二指肠 5-羟色胺 

分 类 号：R245.91[医药卫生—针灸推拿学]