p53+/-基因敲除小鼠的脏器质量及血液学参数测定  

Determination of Hematological and Visceral Weight Parameters of p53+/- Gene Knockout Mice

作　　者：刘甦苏[1] 王洪[1] 魏杰[1] 周舒雅[1] 吴曦[1] 王辰飞[1] 李芳芳[1] 左琴[1] 岳秉飞[1] 范昌发[1] 

机构地区：[1]中国食品药品检定研究院,北京100050

出　　处：《实验动物科学》2016年第5期52-56,60,共6页Laboratory Animal Science

基　　金：中国食品药品检定研究院中青年发展研究基金(No.2015C2)

摘　　要：目的测定自主建立的p53+/-基因敲除小鼠的脏器及血液生理生化指标,并比较周龄、性别对各项指标的影响。方法选取4周龄和8周龄的p53+/-基因敲除小鼠各20只,雌雄各半,测定其主要脏器质量及血液生理指标。结果不同周龄和性别的p53+/-基因敲除小鼠部分脏器质量和血生理生化有差异性。4周龄和8周龄小鼠在体质量等这21项指标上都有差异显著性(P<0.01),且在左肾上腺等8项指标上差异存在统计学意义(P<0.05)。4周龄的雌、雄小鼠在RDW、总胆固醇(CHO)这2项指标上差异存在显著性(P<0.01),仅肺这1项指标上差异有统计学意义(P<0.05),其余指标之间无明显差异;而8周龄雌雄小鼠在体质量等16项指标上雌雄间都有显著性差异(P<0.01),在HCT等9项指标上存在差异(P<0.05)。结论本文测定并比较了不同周龄及性别的p53+/-基因敲除小鼠的脏器及血液生理生化指标,为该模型的合理利用及商业化供应提供了背景数据。Objective To determine the normal range of hematological and visceral weight parameters of 4-week and 8-week old,male and female p53 ＋/-gene knockout mice and analysis the influence of gender and week age. Method Body weight of 4-week old and 8-week old p53 ＋/-gene knockout mice（ half male and half female,n = 20 in each group） were measured,then were sacrificed and the main viscera were weighted,and blood samples were collected to test the physiological and biochemical parameters. Result Some of the organ weights,blood physiological and biochemical parameters of different ages and sexes were significant differences. When the parameters of 4-week old and 8-week old Trp53＋/- mice were compared,there were significant differences in21 parameters（ body weight,heart,liver,left kidney,right kidney,left testis,right testis,WBC,RBC,HGB,HCT,MCV,MCHC,RDW,LYM,AST,ALB,ALP,CREA and Tbili）（ P 〈 0. 01）,and P 〈 0. 05 in 8parameters（ left adrenal,right adrenal,thymus,left ovary,right ovary,LYM%,NEUT% and TP）. When male and female 4-week Trp53＋/- mice were compared,there were significant differences only in 2 parameters（ RDW and CHO）（ P 〈 0. 01）,and P 〈 0. 05 for lung weight. For male and female 8-week old Trp53＋/- mice,there were significant differences in 16 parameters（ body weight,heart,liver,left kidney,right kidney,WBC,RBC,HGB,MCV,MCHC,RDW,LYM,AST,CA,P and CHO）（ P 〈 0. 01）,and P 〈 0. 05 in 9 parameters（ HCT,PLT,PCT,LYM%,NEUT%,ALB,ALP,BUN and GLU）. Conclusion The normal range of hematological and visceral weight parameters of 4-week and 8-week old male and female p53 ＋/-gene knockout mice are determined. Our study provides a background data for rational use and application of the model.

关 键 词：p53+/-基因敲除小鼠 脏器质量 血液生理生化指标 

分 类 号：Q819[生物学—生物工程]