三重复合诱变选育林可霉素高产菌株  

Triple Breeding of Lincomycin Producing Strain

作　　者：毕国东 牛春 黄文福 张萍 BI Guo - dong NIU Chun HUANG Wen - fu ZHANG Ping(Terry Pharmaceutical Co Ltd ,Ningxia Institute of Bacteria, Yinchuan 750100, China)

机构地区：[1]宁夏泰瑞制药股份有限公司技术研发中心,银川750100

出　　处：《中国兽药杂志》2016年第11期22-26,共5页Chinese Journal of Veterinary Drug

摘　　要：以提高林可霉素的生产水平为目的,将林可霉素产生菌LK15-012先用0.6%甲基磺酸乙酯(EMS)处理3 h,然后常压室温等离子体(ARTP-200W)照射90 s、最后紫外线(UV)照射50 s。结果表明:致死率为98.5%,正突变率为71.8%。最终获得2株高产突变株LK16-032和LK16-049,摇瓶效价比出发菌株LK15-012提高了42.50%和35.25%。将两株突变株分别通过100 L小试、10 t中试和60 t大生产进行发酵验证,发酵效价明显高于生产菌株。采用EMS-ARTP-UV三重复合诱变明显提高了林可霉素的生产水平。In order to improve the level of production treated with 0.6% ethyl methyl sulfonic acid （EMS）3 200w） at room temperature irradiation of 90 s, finally of lincomycin, clindamycin producing bacteria LK15 - 012 h, the second with atmospheric pressure plasma （ARTP - by ultraviolet （UV） radiation 50 s. The results showed that the mortality rate was 98.5%, the positive mutation rate is 71.8%. Won 2 strains of high yield mutant strains LK16 -032and LK16 - 049, shake flask titer than the starting strain LK15 - 012 increased by 42.50% and 35.25%. Will be high yield mutant strain respectively through the test in 100 L, 10 t pilot and 60 t fermentation carried out to verify the mass production, significantly higher than the production strains fermentation titer. The EMS -ARTP -UV triple compound mutagenesis lincomycin production levels are increased obviously.

关 键 词：林可霉素 EMS-ARTP-UV三重复合诱变 高产突变株 

分 类 号：S859.796[农业科学—临床兽医学]