木犀草素联合庆大霉素对耐药化脓隐秘杆菌抑制作用的研究  

Study on Inhibiting Effects of Luteolin Combined with Gentamicin Against Drug-resistant Trueperella pyogenes

作　　者：宋雪娇[1] 刘耀川[1] 高翔[1] 孙婷婷[1] 田春莲[1] 赵敬翠 张德显[1] 刘明春[1] SONG Xue-jiao LIU Yao-chuan GAO Xiang SUN Ting-ting TIAN Chun-lian ZHAO Jing-cui ZHANG De-xian LIU Ming-chun(College of Animal Husbandary and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, China Jianping Supervision Institute of Animal ProductSafety,Jianping,Liaoning 122400, China)

机构地区：[1]沈阳农业大学畜牧兽医学院,沈阳110866 [2]建平县畜产品安全监督所,辽宁建平122400

出　　处：《中国兽药杂志》2016年第11期54-58,共5页Chinese Journal of Veterinary Drug

基　　金：国家自然科学基金项目(31201954;31572564)

摘　　要：为探究木犀草素联合庆大霉素对耐药化脓隐秘杆菌的抑制作用，采用微量肉汤稀释法分别测定木犀草素和庆大霉素对34株化脓隐秘杆菌的最小抑菌浓度（MIC）。选取对庆大霉素耐药的化脓隐秘杆菌，通过棋盘法测定两种药物联合抑菌效果。经亚抑菌浓度木犀草素作用耐药菌株，比较木犀草素作用前后化脓隐秘杆菌对庆大霉素的耐药性变化情况。结果表明，木犀草素对化脓隐秘杆菌的MIC值为39～156μg/mL，与庆大霉素联合的作用为不相关；耐药菌经亚抑菌浓度木犀草素（1／4MIC）作用36h后，庆大霉素对耐药菌的MIC值下降2～32倍，且原菌株耐药水平越高，MIC下降倍数越高。提示木犀草素具有良好抗菌活性，且能够逆转化脓隐秘杆菌耐药性。To explore the antibacterial effects of luteolin combined with gentamicin, the minimum inhibitory concentrations （MICs） of luteolin and gentamicin against 34 strains of Trueperella pyogenes （ T. Fyogenes） were tested by broth microdilution method. The gentamicin resistance isolates were selected to determine the co - antibacterial effects between luteolin and gentamicin by the chessboard method. The drug -resistant levels among these isolates were observed after treating with luteolin at a sub -inhibitory concentration. The results indicated that the MIC of luteolin ranged from 39 to 156μg/mL, meanwhile there was no co -effects against T. pyogenes between luteolin and gentamicin. The MICs of these resistant isolates become 1/2 to 1/32 after treating 36 h with a sub -inhibitory concentration （1/4MIC） of luteolin, the MICs decreased significantly as the antibiotic resistant levels become higher.The results show that luteolin has a good antibacterial activity and it can reverse the drug resistance of T. pyogenes.

关 键 词：木犀草素 化脓隐秘杆菌 药物联合 抗菌作用 逆转耐药 

分 类 号：S852.61[农业科学—基础兽医学]