精氨酸双糖苷对脂多糖诱导的巨噬细胞分泌炎症因子的影响  

Effects of Arginyl-Fructosyl-Glucose on the Secretion of Inflammatory Cytokines in Macrophages Induced by Lipopolysaccharide

作　　者：高铭彤 王佳奇[1] 陈凯[1] 李莹[1] 李婧毓 刘一桐 丁传波[1] 刘文丛[1] 郑毅男[1] GAO Ming -tong WANG Jia- qi CHEN Kai LI Ying LI Jing -yu LIU Yi -tong DING Chuan - bo LIU Wen - cong ZHENG Yi - nan(College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China)

机构地区：[1]吉林农业大学中药材学院,长春130118

出　　处：《中国兽药杂志》2016年第11期65-69,共5页Chinese Journal of Veterinary Drug

基　　金：科技型中小企业创新创业基金项目(20160308002YY)

摘　　要：为研究精氨酸双糖苷(AFG)体外的抗炎机制,以脂多糖(LPS)刺激小鼠单核巨噬细胞(RAW264.7)作为炎症模型,用精氨酸双糖苷(AFG)的低、中、高(5、10、20 mg/L)三个剂量进行干预后,MTT法测定细胞毒性作用,Griess法测定一氧化氮(NO)生成量,ELISA法检测细胞上清液中白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)及前列腺素E2(PGE2)的分泌量。结果表明:AFG的三个不同剂量对RAW264.7细胞无抑制作用(P>0.05),各浓度给药组的NO、IL-1β、IL-6、TNF-α和PGE2含量与LPS刺激模型组相比较都显著降低(P<0.01),推想AFG的抗炎活性可能是通过抑制NO和PGE2等炎症介质释放,降低IL-1β、IL-6和TNF-α等炎症因子的含量而发挥了作用。In this paper, we studied the anti - inflammatory mechanism of arginyl - fructosyl - glucose （AFG） in vitro. The inflammation model, which was established based on the mouse monocyte- macrophage cells （RAW264.7） stimulated by lipopolysaccharide （ LPS）, is intervened by three doses of AFG, low, medium and high （5, 10, 20 mg/L）. After intervention, MTI＂ approach was used to access cytotoxicity; Griess approach is used to determine the amount of nitric oxide （NO） ; and ELISA was used to evaluate the secretion of interleukin - 1 β （ IL - 1β ）, interleukin - 6 （ IL - 6 ）, tumor necrosis factor - α（ TNF - α ） and pros in cell supernatants. The results that： The three doses of AFG have no inhibition effect taglandin E2 （ PGE2 ） on RAW264.7 cells （P 〉 0.05）. In the control group, all contents of NO, IL - 1β, IL - 6, TNF -α than placebo group （LPS model） （P 〈0.01 ）. The mechanism of AFG in anti contributed by inhibiting the release of inflammatory mediators of NO and PGE2, IL- 6, TNF -α and other inflammatory factors. oL and PGE2 are significant less -inflammatory activity may be reducing the amount of IL - 1β,

关 键 词：精氨酸双糖苷 小鼠RAW264.7细胞 抗炎活性 炎症因子 

分 类 号：Q53[生物学—生物化学]